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Our Abpromise guarantee covers the use of ab86497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
ab86497 staining GAVARAPL1 in human PC3 prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour. A CF568-conjugated donkey anti-rabbit IgG polyclonal (1/500 was used as the secondary antibody.
This image is courtesy of an anonymous AbreviewBlocking step: 5% Milk for 1 hour at room temperature
Immunohistochemistry analysis of formalin-fixed,paraffin-embedded Human brain tissue labeling GABARAPL1 with ab86497 at a concentration 5.0ug/ml