製品の概要

  • 製品名Anti-GABARAP antibody [EPR4805]
    GABARAP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4805] to GABARAP
  • 特異性ab109364 will also recognize related targets GABARAPL1 and GABARAPL2: GABARAPL1: pBLAST 100% immunogen homology AND Abreview 38385 WB data GABARAPL2: pBLAST 93% homology (only 1aa gap of 14aa) AND Abreview 38385 WB data
  • アプリケーション適用あり: Flow Cyt, WB, IHC-P, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Rat, Human, Silk worm (Bombyx mori)
  • 免疫原

    Synthetic peptide, corresponding to residues in Human GABARAP.

  • ポジティブ・コントロール
    • Fetal kidney lysate, fetal brain lysate, SH-SY5Y cell lysate, HepG2 cell lysate, human brain tissue, HeLa cells.
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P 1/500 - 1/1000. Antigen retriecal is recommended
ICC/IF 1/100 - 1/500.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
    • 組織特異性Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
    • 配列類似性Belongs to the ATG8 family.
    • 翻訳後修飾The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
    • 細胞内局在Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATG8A antibody
      • FLC 3B antibody
      • FLC3B antibody
      • FLJ25768 antibody
      • GABA(A) receptor associated protein antibody
      • GABA(A) receptor-associated protein antibody
      • GABARAP a antibody
      • GABARAP antibody
      • Gamma aminobutyric acid receptor associated protein antibody
      • Gamma-aminobutyric acid receptor-associated protein antibody
      • GBRAP_HUMAN antibody
      • MGC120154 antibody
      • MGC120155 antibody
      • MM 46 antibody
      • MM46 antibody
      see all

    Anti-GABARAP antibody [EPR4805] 画像

    • ab109364 staining GABARAP in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling GABARAP with purified ab109364 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    • All lanes : Anti-GABARAP antibody [EPR4805] (ab109364) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 14 kDa
    • ab109364, at 1/500, staining GABARAP in Human brain tissue by immunohistochemistry.
    • ab109364, at 1/100, staining HeLa cells by immunofluorescence.

    Anti-GABARAP antibody [EPR4805] (ab109364) 使用論文

    This product has been referenced in:
    • Cappelletti C  et al. Autophagy, inflammation and innate immunity in inflammatory myopathies. PLoS One 9:e111490 (2014). Radioimmunoprecipitation ; Human . Read more (PubMed: 25365350) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Human Tissue lysate - whole (skeletal muscle tissue)
    Gel Running Conditions Reduced Denaturing (4-12%)
    Loading amount 20 µg
    Specification skeletal muscle tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 12 2015

    Application Western blot
    Loading amount 40 µg
    Gel Running Conditions Reduced Denaturing (12%)
    Sample Silk worm (Bombyx mori) Tissue lysate - whole (Midgut)
    Specification Midgut
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Sep 02 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (13%)
    Sample Human Cell lysate - whole cell (SH-SY5Y cells stably expressing GFP, GFP-GABARAP,)
    Specification SH-SY5Y cells stably expressing GFP, GFP-GABARAP,
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Dr. George Allen

    Verified customer

    投稿 Sep 26 2013

    Application IHC - Wholemount
    Sample Fruit fly (Drosophila melanogaster) Tissue (fat body)
    Specification fat body
    Username

    Abcam user community

    Verified customer

    投稿 Sep 19 2013

    Thank you very much for your interest our AbTrial program.



    I performed a comparison of the immunogens for our GABARAP antibodies, and the identity between the immunogen of ab109364 and the silkworm protein looks very promising with ...

    Read More

    Thank you for your reply.

    I wish you good luck with the live reporter experiments.

    I will be happy to issue the discount code for you now, but I think it would be better to do this when you know that you will use it. The discount co...

    Read More

    Thank you very much for your interest in our antibodies.

    According to a quick blast check the immunogen of ab1398 has a homology with Drosophila melanogaster of 82%. We generally expect cross reaction of an antibody from 85% homology and mor...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"