Anti-GABARAP + GABARAPL1 + GABARAPL3 抗体 [EPR18862] (ab191888)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18862] to GABARAP + GABARAPL1 + GABARAPL3
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
製品の概要
-
製品名
Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862]
GABARAP+GABARAPL1+GABARAPL3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR18862] to GABARAP + GABARAPL1 + GABARAPL3 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Rat, Human, Recombinant fragment -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Human GABARAPL1 full length recombinant protein; Recombinant human GABARAPL3 full length protein; Recombinant human GABARAP full length protein; Human brain lysate; HeLa and HepG2 whole cell lysates; Mouse brain, kidney and spleen lysates; Rat brain and kidney lysates. IP: Mouse brain lysate
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR18862 -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab191888の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP |
1/150.
|
|
WB |
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
|
特記事項 |
---|
IP
1/150. |
WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa). |
ターゲット情報
-
細胞内局在
GABARAP: Endomembrane system. Cytoplasm > cytoskeleton. Golgi apparatus membrane. Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules. GABARAPL1: Cytoplasm > cytoskeleton. Cytoplasmic vesicle membrane. Endoplasmic reticulum. Golgi apparatus. Cytoplasmic vesicle > autophagosome. GABARAPL3: Cytoplasmic; Cytoskeleton; Microtubule -
参照データベース
- Entrez Gene: 11337 Human
- Entrez Gene: 23710 Human
- Entrez Gene: 23766 Human
- Entrez Gene: 56486 Mouse
- Entrez Gene: 57436 Mouse
- Entrez Gene: 58974 Rat
- Entrez Gene: 689161 Rat
- Omim: 605125 Human
see all
画像
-
Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/1000 dilution + Human GABARAPL1 full length recombinant protein at 0.02 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Human GABARAPL1 full length recombinant protein contains aa1-117 with a GST-Tag.
-
All lanes : Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/5000 dilution
Lane 1 : Recombinant human GABARAPL2 full length protein
Lane 2 : Recombinant human GABARAPL3 full length protein
Lane 3 : Recombinant human GABARAP full length protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1 and 2: 5 seconds; Lane 3: 3 minutes.
All three recombinant human full length proteins contain aa1-117 with an N-terminal His-Tag®. This product showed higher reactivity with GABARAPL1 and GABARAPL3, and lower reactivity with GABARAP.
-
Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/1000 dilution + Human brain tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The observed MW is a little higher than predicted one (14KD), which is similar with the published literatures (PMID: 16650615 & 21862879).
-
All lanes : Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time:30 seconds
The observed MW is a little higher than predicted one (14KD), which is similar with the published literatures (PMID: 16650615 & 21862879).
-
All lanes : Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The observed MW is a little higher than predicted one (14KD), which is similar with the published literatures (PMID: 16650615 & 21862879).
-
GABARAP + GABARAPL1 + GABARAPL3 was immunoprecipitated from 1 mg of Mouse brain lysate with ab191888 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab191888 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse brain lysate, 10µg (Input).
Lane 2: ab191888 IP in Mouse brain lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab191888 in Mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (1)
ab191888 は 1 報の論文で使用されています。
- Shi J et al. A genome-wide CRISPR screen identifies WDFY3 as a regulator of macrophage efferocytosis. Nat Commun 13:7929 (2022). PubMed: 36566259