Anti-GABA Transporter 1 / GAT 1 抗体 [EPR12998] (ab177483)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998]
GABA Transporter 1 / GAT 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human GABA Transporter 1/ GAT 1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P30531 -
ポジティブ・コントロール
- Fetal brain lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12998 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177483の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 67 kDa. |
ターゲット情報
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機能
Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. -
配列類似性
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily. -
ドメイン
The PDZ domain-binding motif is involved in the interaction with MPP5. -
細胞内局在
Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons. - Information by UniProt
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参照データベース
- Entrez Gene: 6529 Human
- Entrez Gene: 232333 Mouse
- Entrez Gene: 79212 Rat
- Omim: 137165 Human
- SwissProt: P30531 Human
- SwissProt: P31648 Mouse
- SwissProt: P23978 Rat
- Unigene: 443874 Human
see all -
別名
- GABATHG antibody
- GABATR antibody
- GABT 1 antibody
see all
画像
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All lanes : Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/10000 dilution
Lane 1 : Human brain lysates unboiled
Lane 2 : Human brain lysates boiled
Lane 3 : Mouse brain unboiled
Lane 4 : Mouse brain boiled
Lane 5 : Rat brain unboiled
Lane 6 : Rat brain boiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time:
Lane 1 to 2: 20 seconds
Lane 3 to 6: 8 secondsWe recommend not to boil the samples after lysis to get desired WB results.
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Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/1000 dilution + Fetal brain lysate at 10 µg
Predicted band size: 67 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab177483 は 4 報の論文で使用されています。
- Jin L et al. Astrocytic SARM1 promotes neuroinflammation and axonal demyelination in experimental autoimmune encephalomyelitis through inhibiting GDNF signaling. Cell Death Dis 13:759 (2022). PubMed: 36055989
- Cai HY et al. Adjusting vascular permeability, leukocyte infiltration, and microglial cell activation to rescue dopaminergic neurons in rodent models of Parkinson's disease. NPJ Parkinsons Dis 7:91 (2021). PubMed: 34625569
- Ren W et al. Slc6a13 deficiency promotes Th17 responses during intestinal bacterial infection. Mucosal Immunol N/A:N/A (2018). PubMed: 30523310
- Maolakuerban N et al. MiR-200c-3p inhibits cell migration and invasion of clear cell renal cell carcinoma via regulating SLC6A1. Cancer Biol Ther 19:282-291 (2018). PubMed: 29394133