Anti-GABA Transporter 1 / GAT 1 抗体 (ab72448)

製品の概要

  • 製品名Anti-GABA Transporter 1 / GAT 1 antibody
    GABA Transporter 1 / GAT 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GABA Transporter 1 / GAT 1
  • アプリケーション適用あり: IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Peptide corresponding to amino acid residues from the C-terminal region of rat GABA Transporter 1/ GAT 1

  • ポジティブ・コントロール
    • rat hippocampal homogenate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, 0.1mg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

ターゲット情報

  • 機能Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • 配列類似性Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
  • ドメインThe PDZ domain-binding motif is involved in the interaction with MPP5.
  • 細胞内局在Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GABATHG antibody
    • GABATR antibody
    • GABT 1 antibody
    • GABT1 antibody
    • GAT-1 antibody
    • GAT1 antibody
    • SC6A1_HUMAN antibody
    • Slc6a1 antibody
    • Sodium and chloride dependent GABA transporter 1 antibody
    • Sodium- and chloride-dependent GABA transporter 1 antibody
    • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
    • Solute carrier family 6 member 1 antibody
    see all

Anti-GABA Transporter 1 / GAT 1 antibody 画像

  • Anti-GABA Transporter 1 / GAT 1 antibody (ab72448) at 1/1000 dilution + rat hippocampal homogenate

    Predicted band size : 67 kDa
    Observed band size : 67 kDa
  • ab72448 staining GABA Transporter 1 / GAT 1 in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/500 in 5% serum) for 16 hours at 4°C. A DyLight��-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

Anti-GABA Transporter 1 / GAT 1 antibody (ab72448) 使用論文

ab72448 has not yet been referenced specifically in any publications.

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Thank you for contacting us. Your credit note/refund ID is I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchas...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Hippocampus)
Permeabilization Yes - 0.1% PBS-Triton X
Specification Hippocampus
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Jan 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain (hippocampus))
Permeabilization No
Specification Brain (hippocampus)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Mr. Yahyah Aman

Verified customer

投稿 Jan 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Mar 04 2013

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"