製品の概要

  • 製品名Anti-GABA Transporter 1 / GAT 1 antibody
    GABA Transporter 1 / GAT 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GABA Transporter 1 / GAT 1
  • アプリケーション適用あり: ICC/IF, WB, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human GABA Transporter 1/ GAT 1.

    (Peptide available as ab74762.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Bladder Tissue Lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab64645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IHC-FoFr 1/1000.

ターゲット情報

  • 機能Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • 配列類似性Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
  • ドメインThe PDZ domain-binding motif is involved in the interaction with MPP5.
  • 細胞内局在Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GABATHG antibody
    • GABATR antibody
    • GABT 1 antibody
    • GABT1 antibody
    • GAT-1 antibody
    • GAT1 antibody
    • SC6A1_HUMAN antibody
    • Slc6a1 antibody
    • Sodium and chloride dependent GABA transporter 1 antibody
    • Sodium- and chloride-dependent GABA transporter 1 antibody
    • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
    • Solute carrier family 6 member 1 antibody
    see all

Anti-GABA Transporter 1 / GAT 1 antibody 画像

  • Anti-GABA Transporter 1 / GAT 1 antibody (ab64645) at 1 µg/ml + Human bladder tissue lysate - soluble fraction (female, 45 years) (ab44654) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 67 kDa
    Observed band size : 67 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • ICC/IF image of ab64645 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64645, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC-FoFr image of GABA Transporter 1 / GAT1 staining on Mouse Hippocampus sections using ab64645(1:1000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

Anti-GABA Transporter 1 / GAT 1 antibody (ab64645) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
Sample Human Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative 10% buffered formalin
Username

Abcam user community

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投稿 Oct 20 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Retina, neurons)
Specification Retina, neurons
Permeabilization Yes - Triton X
Fixative Paraformaldehyde
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投稿 Oct 03 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Username

Dr. Sophie Pezet

Verified customer

投稿 May 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"