製品の概要

  • 製品名Anti-GABA Receptor Epsilon antibody
    GABA Receptor Epsilon 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GABA Receptor Epsilon
  • アプリケーション適用あり: IHC-FoFr, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat
  • 免疫原

    A region within synthetic peptide: KNSWKLFQFD FTGVSNKTEI ITTPVGDFMV MTIFFNVSRR FGYVAFQNYV, corresponding to intracellular amino acids 237-286 of Human GABA Receptor Epsilon

  • ポジティブ・コントロール
    • Placenta lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab35971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 21084677
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • 組織特異性Expressed in many tissues. Highest levels of expression in adult heart and placenta.
  • 配列類似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRE sub-subfamily.
  • 細胞内局在Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GABA(A) receptor epsilon antibody
    • GABA(A) receptor subunit epsilon antibody
    • Gabre antibody
    • Gamma aminobutyric acid (GABA) A receptor epsilon antibody
    • Gamma aminobutyric acid receptor subunit epsilon antibody
    • Gamma-aminobutyric acid receptor subunit epsilon antibody
    • GBRE_HUMAN antibody
    see all

Anti-GABA Receptor Epsilon antibody 画像

  • ICC/IF image of ab35971 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab35971, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab35971 staining in human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab35971, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemical analysis of male, virgin female, G17 pregnant, and 30 d post-partum rat medullary neurons, staining GABA Receptor Epsilon with ab35971 at 1/1000 dilution.
  • Anti-GABA Receptor Epsilon antibody (ab35971) (0.2-1 ug/ml) + Human Placenta

    Predicted band size : 42 kDa

Anti-GABA Receptor Epsilon antibody (ab35971) 使用論文

This product has been referenced in:
  • Fatemi SH  et al. Expression of GABAA a2-, ß1- and ?-receptors are altered significantly in the lateral cerebellum of subjects with schizophrenia, major depression and bipolar disorder. Transl Psychiatry 3:e303 (2013). WB ; Human . Read more (PubMed: 24022508) »
  • Hengen KB  et al. Increased GABA(A) Receptor e-Subunit Expression on Ventral Respiratory Column Neurons Protects Breathing during Pregnancy. PLoS One 7:e30608 (2012). IHC-FoFr ; Rat . Read more (PubMed: 22303446) »

See all 3 Publications for this product

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