Anti-GABA B Receptor 2 (phospho S893) 抗体 [EP2318Y] (ab68426)

製品の概要

  • 製品名Anti-GABA B Receptor 2 (phospho S893) antibody [EP2318Y]
    GABA B Receptor 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2318Y] to GABA B Receptor 2 (phospho S893)
  • 特異性Detects GABA B Receptor 2 only when phosphorylated on serine 893.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide corresponding to residues surrounding serine 893 of human GABA B Receptor 2.

  • ポジティブ・コントロール
    • Brain
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
WB 1/500 - 1/1000. Detects a band of approximately 105 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The use of an HRP/AP polymerized secondary antibody is recommended.

  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
    • 組織特異性Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.
    • 配列類似性Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
    • ドメインAlpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.
    • 細胞内局在Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
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      • GABABR 2 antibody
      • GABABR2 antibody
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      • GABBR 2 antibody
      • Gabbr2 antibody
      • GABR2_HUMAN antibody
      • Gamma aminobutyric acid B receptor 2 antibody
      • Gamma aminobutyric acid GABA B receptor 2 antibody
      • Gamma aminobutyric acid type B receptor subunit 2 antibody
      • Gamma-aminobutyric acid type B receptor subunit 2 antibody
      • Gb 2 antibody
      • Gb2 antibody
      • GH07312 antibody
      • GPR 51 antibody
      • GPR51 antibody
      • GPRC 3B antibody
      • GPRC3B antibody
      • HG 20 antibody
      • HG20 antibody
      • HRIHFB2099 antibody
      • Metabotropic GABA B receptor subtype 2 antibody
      • OTTHUMP00000021776 antibody
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    Anti-GABA B Receptor 2 (phospho S893) antibody [EP2318Y] 画像

    • All lanes : Anti-GABA B Receptor 2 (phospho S893) antibody [EP2318Y] (ab68426) at 1/1000 dilution

      Lane 1 : Human brain lysate - untreated
      Lane 2 : Human brain lysate - treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

      Observed band size : 106 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of phosphorylated GABA B Receptor 2 expression on paraffin-embedded Human brain tissue using 1/100 ab68426. Heat mediated antigen retrieval was performed, and an HRP-conjugated polymerized secondary antibody was used.

    Anti-GABA B Receptor 2 (phospho S893) antibody [EP2318Y] (ab68426) 使用論文

    ab68426 has not yet been referenced specifically in any publications.

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