Anti-GABA B Receptor 2/GABBR2 (phospho S893) 抗体 [EP2318Y] (ab68426)
Key features and details
- Rabbit monoclonal [EP2318Y] to GABA B Receptor 2/GABBR2 (phospho S893)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-GABA B Receptor 2/GABBR2 (phospho S893) antibody [EP2318Y]
GABA B Receptor 2/GABBR2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2318Y] to GABA B Receptor 2/GABBR2 (phospho S893) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human brain tissue. IHC-P: Human brain tissue.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP2318Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68426の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 105 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended. |
特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 105 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended. |
ターゲット情報
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機能
Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. -
組織特異性
Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle. -
配列類似性
Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily. -
ドメイン
Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9568 Human
- Entrez Gene: 242425 Mouse
- Entrez Gene: 83633 Rat
- Omim: 607340 Human
- SwissProt: O75899 Human
- SwissProt: Q80T41 Mouse
- SwissProt: O88871 Rat
- Unigene: 198612 Human
see all -
別名
- BcDNA:GH07312 antibody
- CG6706 antibody
- CT20836 antibody
see all
画像
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All lanes : Anti-GABA B Receptor 2/GABBR2 (phospho S893) antibody [EP2318Y] (ab68426) at 1/1000 dilution
Lane 1 : Human brain lysate - untreated
Lane 2 : Human brain lysate - treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution
Observed band size: 106 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of phosphorylated GABA B Receptor 2/GABBR2 expression on paraffin-embedded Human brain tissue using ab68426 at 1/100 dilution. Heat mediated antigen retrieval was performed, and an HRP-conjugated polymerized secondary antibody was used.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab68426 は 1 報の論文で使用されています。
- Kurata S et al. Exogenous gamma-aminobutyric acid addition enhances porcine sperm acrosome reaction. Anim Sci J 93:e13744 (2022). PubMed: 35699686