製品の概要

  • 製品名Anti-GABA B Receptor 2 antibody
    GABA B Receptor 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GABA B Receptor 2
  • 特異性ab52248 detects endogenous levels of total GABA B Receptor 2 protein.
  • アプリケーション適用あり: IHC-FoFr, IHC (PFA fixed), WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from human GABA B receptor.

  • ポジティブ・コントロール
    • HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration.
IHC (PFA fixed) 1/300.
WB 1/300 - 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
  • 組織特異性Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.
  • 配列類似性Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
  • ドメインAlpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.
  • 細胞内局在Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
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    • Gamma aminobutyric acid GABA B receptor 2 antibody
    • Gamma aminobutyric acid type B receptor subunit 2 antibody
    • Gamma-aminobutyric acid type B receptor subunit 2 antibody
    • Gb 2 antibody
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    • HG 20 antibody
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Anti-GABA B Receptor 2 antibody 画像

  • All lanes : Anti-GABA B Receptor 2 antibody (ab52248) at 1/300 dilution

    Lane 1 : HeLa cell extract; without peptide
    Lane 2 : HeLa cell extract; with peptide


    Predicted band size : 106 kDa
    Observed band size : 106 kDa
  • Immunohistochemistical detection of GABA B Receptor 2 antibody (ab52248) in PFA fixed Rat Dorsal root ganglion sections. ab52248 was used at 1/300 and incubated for 60 hours @ 4°C in PBS-T 0.3%. Secondary antibody: anti IgG Rabbit Conjugation: Alexa Fluor® 488 (1/1000).

Anti-GABA B Receptor 2 antibody (ab52248) 使用論文

This product has been referenced in:
  • Thibault K  et al. Molecular mechanisms underlying the enhanced analgesic effect of oxycodone compared to morphine in chemotherapy-induced neuropathic pain. PLoS One 9:e91297 (2014). WB, IHC-Fr, IHC-FrFl ; Rat . Read more (PubMed: 24618941) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Dorsal root ganglion)
Specification Dorsal root ganglion
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Karine Thibault

Verified customer

投稿 Oct 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"