製品の概要

  • 製品名Anti-GABA B Receptor 1 antibody
    GABA B Receptor 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GABA B Receptor 1
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthesized peptide derived from internal of human GABA B Receptor 1

  • ポジティブ・コントロール
    • extracts from K562 cells; HeLa cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab75239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 108 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen.
    Isoform 1E function may be to regulate the availability of functional GABA-B-R1A/GABA-B-R2 heterodimers by competing for GABA-B-R2 dimerization. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
  • 組織特異性Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland.
  • 配列類似性Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
    Contains 2 Sushi (CCP/SCR) domains.
  • ドメインAlpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably play a role in the specificity for G-protein coupling.
  • 細胞内局在Secreted and Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes (By similarity). Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ271M21.1.1 antibody
    • dJ271M21.1.2 antibody
    • FLJ92613 antibody
    • GABA-B receptor 1 antibody
    • GABA-B-R1 antibody
    • GABA-BR1 antibody
    • GABAB R1 antibody
    • GABAB subunit 1c antibody
    • GABABR1 antibody
    • GABBR1 3 antibody
    • GABBR1 antibody
    • GABR1_HUMAN antibody
    • Gamma aminobutyric acid (GABA) B receptor 1 antibody
    • Gamma-aminobutyric acid type B receptor subunit 1 antibody
    • Gb1 antibody
    • GPRC3A antibody
    • Seven transmembrane helix receptor antibody
    see all

Anti-GABA B Receptor 1 antibody 画像

  • All lanes : Anti-GABA B Receptor 1 antibody (ab75239) at 1/500 dilution

    Lane 1 : extracts from K562 cells
    Lane 2 : extracts from K562 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 108 kDa
    Observed band size : 108 kDa
  • Left panel: immunofluorescence analysis of HeLa cells with 1/500 ab75239. Right panel: same, after preincubation with the immunizing peptide.

Anti-GABA B Receptor 1 antibody (ab75239) 使用論文

This product has been referenced in:
  • Cuny H  et al. ?-Aminobutyric Acid Type B (GABAB) Receptor Expression Is Needed for Inhibition of N-type (Cav2.2) Calcium Channels by Analgesic a-Conotoxins. J Biol Chem 287:23948-57 (2012). ICC/IF . Read more (PubMed: 22613715) »

See 1 Publication for this product

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The immunogen is in fact located in a cytoplasmic region near the C-terminus. If you fix your cells with PFA, you will need to permeabilize the cells with detergent (for instance, with 0.1% Triton X-100) before a...

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Thanks so much for sending the results and for keeping me updated. I'm sorry to see that the antibody is still picking up so many bands, however you may be correct that some of the bands are isoforms of the target protein. In order for the Abre...

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