製品の概要

  • 製品名Anti-GABA B Receptor 1 antibody
    GABA B Receptor 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to GABA B Receptor 1
  • アプリケーション適用あり: WB, IHC-FoFr, IHC-Fr, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment: AVYIGALFPM SGGWPGGQAC QPAVEMALED VNSRRDILPD YELKLIHHDS KCDPGQATKY LYELLYNDPI KIILMPGCSS VSTLVAEAAR MWNLIVLSYG , corresponding to amino acids 52-151 of Human GABA B Receptor 1

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 108 kDa.
IHC-FoFr 1/1000.
IHC-Fr 1/1000.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen.
    Isoform 1E function may be to regulate the availability of functional GABA-B-R1A/GABA-B-R2 heterodimers by competing for GABA-B-R2 dimerization. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
  • 組織特異性Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland.
  • 配列類似性Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
    Contains 2 Sushi (CCP/SCR) domains.
  • ドメインAlpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably play a role in the specificity for G-protein coupling.
  • 細胞内局在Secreted and Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes (By similarity). Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ271M21.1.1 antibody
    • dJ271M21.1.2 antibody
    • FLJ92613 antibody
    • GABA-B receptor 1 antibody
    • GABA-B-R1 antibody
    • GABA-BR1 antibody
    • GABAB R1 antibody
    • GABAB subunit 1c antibody
    • GABABR1 antibody
    • GABBR1 3 antibody
    • GABBR1 antibody
    • GABR1_HUMAN antibody
    • Gamma aminobutyric acid (GABA) B receptor 1 antibody
    • Gamma-aminobutyric acid type B receptor subunit 1 antibody
    • Gb1 antibody
    • GPRC3A antibody
    • Seven transmembrane helix receptor antibody
    see all

Anti-GABA B Receptor 1 antibody 画像



  • Predicted band size : 108 kDa
    GABA B Receptor 1 antibody (ab55051) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • ICC/IF image of ab55051 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55051, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistical detection of GABA B Receptor 1 antibody (ab55051) in PFA perfusion-fixed rat spinal cord section. ab55051 was used at 1/500, incubated for 18 hours @ 20°C in PBS-T 0.3%.  Secondary antibody: Alexa Fluor® 546 anti-IgG Mouse (1/1000).

    See Abreview

  • ab55051 staining rat brain (hippocampus) sections by IHC-Fr. The sections were  fixed with 4% paraformaldehyde in 0.3% PBS-Tween for 1h. Staining with ab5505 at a 1/1000 dilution in PBS-Triton (0.3%) with 0.02% azide was performed for 24h at 24°C. A donkey anti-rabbit Alexa568 polyclonal antibody at 1/1000 was used as the secondary antibody.  DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. GABA-B R1 expressed in the hippocampal neurons.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab55051 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55051, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with L-Glutamate (ab120049), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of L-Glutamate, as described in literature.
    The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120049 (L-Glutamate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with NMDA (ab120052), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of NMDA, as described in literature.
    The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120052 (NMDA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-GABA B Receptor 1 antibody (ab55051) 使用論文

This product has been referenced in:
  • Giachino C  et al. GABA suppresses neurogenesis in the adult hippocampus through GABAB receptors. Development 141:83-90 (2014). Mouse . Read more (PubMed: 24284211) »
  • Thibault K  et al. Molecular mechanisms underlying the enhanced analgesic effect of oxycodone compared to morphine in chemotherapy-induced neuropathic pain. PLoS One 9:e91297 (2014). IHC-FrFl ; Rat . Read more (PubMed: 24618941) »

See all 6 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Rat brain-Hippocampus)
Specification Rat brain-Hippocampus
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Mar 02 2012

Thanks so much for sending the results and for keeping me updated. I'm sorry to see that the antibody is still picking up so many bands, however you may be correct that some of the bands are isoforms of the target protein. In order for the Abre...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 30 min TBS + 0.5% Triton
Username

Abcam user community

Verified customer

投稿 Mar 18 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Spinal cord)
Specification Spinal cord
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Karine Thibault

Verified customer

投稿 Oct 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"