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Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab166604 as a replacement.
Our Abpromise guarantee covers the use of ab55051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 108 kDa.|
|Flow Cyt||Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 10 µg/ml.|
ab55051 staining rat brain (hippocampus) sections by IHC-Fr. The sections were fixed with 4% paraformaldehyde in 0.3% PBS-Tween for 1h. Staining with ab5505 at a 1/1000 dilution in PBS-Triton (0.3%) with 0.02% azide was performed for 24h at 24°C. A donkey anti-rabbit Alexa568 polyclonal antibody at 1/1000 was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. GABA-B R1 expressed in the hippocampal neurons.
Immunohistochemistical detection of GABA B Receptor 1 antibody (ab55051) in PFA perfusion-fixed rat spinal cord section. ab55051 was used at 1/500, incubated for 18 hours @ 20°C in PBS-T 0.3%. Secondary antibody: Alexa Fluor® 546 anti-IgG Mouse (1/1000).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"