製品の概要

  • 製品名
  • 製品の詳細
    Guinea pig polyclonal to GABA
  • 由来種
    Guinea pig
  • 特異性
    Recognizes GABA. Staining was blocked by preabsorbing with 100uM GABA conjugated to glutaraldehyde. 500uM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining.
  • アプリケーション
    適用あり: Electron Microscopy, ICC/IF, IHC-FoFr, IHC-Fr, ELISAmore details
  • 種交差性
    交差種: Species independent
  • 免疫原

    Chemical/ Small Molecule conjugated to KLH via glutaraldehyde.

  • 特記事項

    Without colchicine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: None
    Constituents: Whole serum
  • 精製度
    Whole antiserum
  • 一次抗体 備考
    Without colchine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab17413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Electron Microscopy Use at an assay dependent concentration. PubMed: 20206640
ICC/IF 1/1000.
IHC-FoFr 1/1000.
IHC-Fr 1/1500.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
  • 細胞内局在
    Cell Membrane, Cytoplasmic, Plasma membrane and Secreted
  • 別名
    • 4 aminobutanoic acid antibody
    • GABA antibody
    • Gamma amino butyric acid antibody
    • Gamma aminobutyric acid antibody

画像

  • Immunohistochemical analysis of rat brain tissue, labelling GABA with ab17413 diluted 1/500. Heat-induced antigen retrieval. DAB staining. Diffuse cytoplasmic staining and axonal staining observed in ventral midbrain neurons. (Note: This antibody produces diffuse cytoplasmic staining of moderate intensity in ventral midbrain, cortical and purkinje neurons in rat brain tissue). 

参考文献

This product has been referenced in:
  • Chang JH  et al. Generation of Functional Dopaminergic Neurons from Reprogramming Fibroblasts by Nonviral-based Mesoporous Silica Nanoparticles. Sci Rep 8:11 (2018). Read more (PubMed: 29311646) »
  • Chan RW  et al. Low-frequency hippocampal-cortical activity drives brain-wide resting-state functional MRI connectivity. Proc Natl Acad Sci U S A 114:E6972-E6981 (2017). IHC . Read more (PubMed: 28760982) »

See all 15 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Cerebral Organoid)
Specification
Human Cerebral Organoid
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Radhika Menon

Verified customer

投稿 Sep 06 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 09 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (LDT brain slices)
Specification
LDT brain slices
Permeabilization
Yes - TritonX
Fixative
PFA/Gluteraldehyde
Username

Jason Teem

Verified customer

投稿 Sep 10 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Sample
Chicken Tissue sections (chicken hindbrain)
Specification
chicken hindbrain
Permeabilization
Yes - 0.1% PBS-TritonX
Fixative
Paraformaldehyde
Username

Karli Montague

Verified customer

投稿 Mar 26 2014


I can confirm that ab17413 anti-GABA antibody is sold as whole antiserum. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentra...

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Thank you for bringing this to our attention. We have not tested the antibody on free-floating sections but your protocol is correct, assuming 10 minutes for permeabilization of these 50um sections was sufficient. We have one other complaint about this...

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Thank you for contacting us.

This antibody has been tested on GABAergic interneurons of mammalian samples, but we do not have testing or reports using hESC.

I hope this information is helpful to you. Please do not hesitate to contact ...

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Thank you for providing some further useful information.

Both antibodies would be suitable for the immunostaining. However, it seems that ab17413 is more popular amongst our customers since it has been characterized in a number of application...

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Thank you for contacting Abcam regarding ab17413. Regarding the tissue fixation, I have confirmed with the laboratory that yes, the combination of 4% paraformaldehyde / glutaraldehyde fixation is typically used in successful IHC staining.  Please f...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case f...

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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