Anti-GABA 抗体 (ab17413)
Key features and details
- Guinea pig polyclonal to GABA
- Suitable for: IHC-P, ICC/IF, IHC-FoFr, IHC-Fr, ELISA
- Reacts with: Species independent
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GABA antibody
GABA 一次抗体 製品一覧 -
製品の詳細
Guinea pig polyclonal to GABA -
由来種
Guinea pig -
特異性
Recognizes GABA. Staining was blocked by preabsorbing with 100uM GABA conjugated to glutaraldehyde. 500uM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining. -
アプリケーション
適用あり: IHC-P, ICC/IF, IHC-FoFr, IHC-Fr, ELISAmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to GABA conjugated to bovine serum albumin (Glutaraldehyde).
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特記事項
Without colchicine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
一次抗体 備考
Without colchine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17413の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/1000.
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IHC-FoFr | (2) |
1/1000.
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IHC-Fr | (2) |
1/1500.
|
ELISA |
Use at an assay dependent concentration.
|
特記事項 |
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IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/1000. |
IHC-FoFr
1/1000. |
IHC-Fr
1/1500. |
ELISA
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell. -
細胞内局在
Cell Membrane, Cytoplasmic, Plasma membrane and Secreted -
別名
- 4 aminobutanoic acid antibody
- GABA antibody
- Gamma amino butyric acid antibody
- Gamma aminobutyric acid antibody
画像
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Immunohistochemical analysis of rat brain tissue, labelling GABA with ab17413 diluted 1/500. Heat-induced antigen retrieval. DAB staining. Diffuse cytoplasmic staining and axonal staining observed in ventral midbrain neurons. (Note: This antibody produces diffuse cytoplasmic staining of moderate intensity in ventral midbrain, cortical and purkinje neurons in rat brain tissue).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (26)
ab17413 は 26 報の論文で使用されています。
- Yoo YM et al. Amylin Protein Expression in the Rat Brain and Neuro-2a Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35457166
- Stumpner A et al. Auditory DUM neurons in a bush-cricket: inhibited inhibitors. J Comp Physiol A Neuroethol Sens Neural Behav Phys 206:793-807 (2020). PubMed: 32656577
- Li C et al. Downregulation of microRNA-29c reduces pain after child delivery by activating the oxytocin-GABA pathway. Mol Med Rep 22:1921-1931 (2020). PubMed: 32705167
- Park SY et al. Distribution of and steroid hormone effects on calbindin-D9k in the immature rat brain. Brain Res Bull 152:225-235 (2019). PubMed: 31357009
- Leroy F et al. A circuit from hippocampal CA2 to lateral septum disinhibits social aggression. Nature 564:213-218 (2018). PubMed: 30518859