製品の概要

  • 製品名Anti-GABA antibody [5A9]
    GABA 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5A9] to GABA
  • 特異性ab86186 is highly specific to GABA and does not react with other amino acid BSA conjugates with the exception of weak reaction against beta alanine BSA conjugate at the highest concentrations used.
  • アプリケーション適用あり: IHC-Fr, IHC-P, ICC/IFmore details
  • 免疫原

    GABA coupled to BSA with glutaraldehyde

  • 特記事項


    ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 一次抗体 備考ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
  • ポリ/モノモノクローナル
  • クローン名5A9
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. (if perfusion fixed)
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
  • 細胞内局在Cell Membrane, Cytoplasmic, Plasma membrane and Secreted
  • 別名
    • 4 aminobutanoic acid antibody
    • GABA antibody
    • Gamma amino butyric acid antibody
    • Gamma aminobutyric acid antibody

Anti-GABA antibody [5A9] 画像

  • ab86186 staining GABA in the Brains of 2-5 days old moths (Manduca) by ICC/IF (Immunocytochemistry/immunofluorescence). The head capsules were fixed with 4% paraformaldehyde, washed in PBS and embedded in Low melting point Agarose and section to 75 μm thickness.Section were blocked with 2% BSA(IgG-free) . Samples were incubated with primary antibody (1/500 in PBS).

    Double labeling against the Ms5HT1A receptor (green) and GABA (magenta) revealed a subset of Ms5HT1A-ir cell bodies co-localizing GABA (arrows). Scale bar = 100 μm.

Anti-GABA antibody [5A9] (ab86186) 使用論文

This product has been referenced in:
  • Dacks AM  et al. A characterization of the Manduca sexta serotonin receptors in the context of olfactory neuromodulation. PLoS One 8:e69422 (2013). IHC-P . Read more (PubMed: 23922709) »

See 1 Publication for this product

Product Wall

Application IHC - Wholemount
Sample Drosophila melanogaster Tissue (Brain)
Specification Brain
Username

Mr. Lee Sun

Verified customer

投稿 Oct 21 2016

GABA is a neurotransmitter and species is not applicable for this biochemical. Therefore any anti GABA antibody should work with samples of wide range of species.

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
Sample Human Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative 10% buffered formalin
Username

Abcam user community

Verified customer

投稿 Oct 23 2014

Thank you for contacting us.

GABA is a neurotransmitter which is a small molecule; because of this species is not applicable for anti GABA antibodies - these antibodies will work irrespective of species.

I hope this information is hel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"