Anti-GABA A Receptor alpha 2 抗体 (ab72445)

製品の概要

  • 製品名
    Anti-GABA A Receptor alpha 2 antibody
    GABA A Receptor alpha 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GABA A Receptor alpha 2
  • 特異性
    ab72445 is specific for GABA A Receptor alpha 2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Cow, Dog, Human, Non human primates, Zebrafish
  • 免疫原

    Fusion protein from the cytoplasmic loop of rat GABA A Receptor alpha 2

  • ポジティブ・コントロール
    • rat brain lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

ターゲット情報

  • 機能
    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • 配列類似性
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA2 sub-subfamily.
  • 細胞内局在
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GABA A receptor subunit alpha 2 antibody
    • GABA antibody
    • GABA(A) receptor subunit alpha 2 antibody
    • GABA(A) receptor subunit alpha-2 antibody
    • GABR A2 antibody
    • GABRA 2 antibody
    • GABRA2 antibody
    • GABRA2 protein antibody
    • Gamma aminobutyric acid (GABA) A receptor alpha 2 antibody
    • Gamma aminobutyric acid A receptor alpha 2 antibody
    • Gamma aminobutyric acid receptor alpha 2 subunit antibody
    • Gamma aminobutyric acid receptor subunit alpha 2 antibody
    • Gamma-aminobutyric acid receptor subunit alpha-2 antibody
    • GBRA2_HUMAN antibody
    see all

画像

  • All lanes : Anti-GABA A Receptor alpha 2 antibody (ab72445) at 1/1000 dilution

    Lane 1 : Wild type Mouse brain lysates
    Lane 2 : GABA A Receptor alpha 2 knockout mouse brain lysate


    Predicted band size : 51 kDa
    Observed band size : 51 kDa

プロトコール

参考文献

This product has been referenced in:
  • Kaneko Y  et al. Oxytocin modulates GABAAR subunits to confer neuroprotection in stroke in vitro. Sci Rep 6:35659 (2016). WB ; Rat . Read more (PubMed: 27767042) »
  • Bates RC  et al. Reduced CHRNA7 expression in C3H mice is associated with increases in hippocampal parvalbumin and glutamate decarboxylase-67 (GAD67) as well as altered levels of GABA(A) receptor subunits. Neuroscience 273:52-64 (2014). Read more (PubMed: 24836856) »

See all 4 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain, Frontal cortex)
Permeabilization
Yes - Triton X-100
Specification
Brain, Frontal cortex
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 29 2017

Thank you for contacting Abcam regarding the immunogen information for ab72445 and ab103707.

Regarding ab103707, the immunogen was generated from a peptide within aa53-83, corresponding to an extracellular domain of the protein. The specific a...

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Thank you for your reply.

There are few protocol variations that may help improve the results that your are getting:

1 - Do not heat/boil your samples. When looking at transmembrane proteins, it is advisable to not heat the sample a...

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Thank you for contacting Abcam.

I would be more than happy to help out in any way that I can. I just have a few more protocol questions for you:

1 - Are you boiling your samples? If so for how long?

2 - What percentage of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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