製品の概要

  • 製品名
    Anti-GAB2 (phospho Y643) antibody
    GAB2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GAB2 (phospho Y643)
  • 特異性
    This antibody detects endogenous levels of GAB2 only when phosphorylated at tyrosine 643.
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthesized phosphopeptide derived from human GAB2 around the phosphorylation site of tyrosine 643 (V-D-YP-V-Q).

  • ポジティブ・コントロール
    • Extracts from Jurkat cells. Human brain tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab78262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/40000.

ターゲット情報

  • 機能
    Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.
  • 配列類似性
    Belongs to the GAB family.
    Contains 1 PH domain.
  • ドメイン
    The SH3-binding motifs mediate interaction with SHC1 and GRB2.
    The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.
  • 翻訳後修飾
    Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
    Dephosphorylated by PTPN11.
  • 細胞内局在
    Cytoplasm. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GAB 2 antibody
    • Gab2 antibody
    • GAB2_HUMAN antibody
    • Grb 2 associated binder 2 antibody
    • GRB 2 associated binding protein 2 antibody
    • Grb2 associated binder 2 antibody
    • GRB2 associated binder 2 pp100 antibody
    • GRB2 associated binding protein 2 antibody
    • GRB2-associated binder 2 antibody
    • GRB2-associated-binding protein 2 antibody
    • Growth factor receptor bound protein 2 associated protein 2 antibody
    • Growth factor receptor bound protein 2-associated protein 2 antibody
    • KIAA0571 antibody
    • p97 antibody
    • PH domain containing adaptor molecule p97 antibody
    • pp100 antibody
    see all

画像

  • All lanes : Anti-GAB2 (phospho Y643) antibody (ab78262) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells, treated with IFN (2500U/ML, 30mins)
    Lane 2 : Extracts from Jurkat cells, treated with IFN (2500U/ML, 30mins) plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab78262 at a 1/50 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

参考文献

This product has been referenced in:
  • Yim JH  et al. Phosphoprotein profiles of candidate markers for early cellular responses to low-dose ?-radiation in normal human fibroblast cells. J Radiat Res 58:329-340 (2017). WB ; Human . Read more (PubMed: 28122968) »

See 1 Publication for this product

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