製品の概要

  • 製品名Anti-GAB2 antibody [EP369Y]
    GAB2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP369Y] to GAB2
  • 特異性This antibody is predicted to detect the splice isoform 2 based on sequence homology.
  • アプリケーション適用あり: WB, ICC/IF, Flow Cytmore details
    適用なし: IHC or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in human Gab2

  • ポジティブ・コントロール
    • PC12 cells; K562 cells; MCF7 cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 74 kDa).
ICC/IF 1/50.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for IHC or IP.
  • ターゲット情報

    • 機能Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.
    • 配列類似性Belongs to the GAB family.
      Contains 1 PH domain.
    • ドメインThe SH3-binding motifs mediate interaction with SHC1 and GRB2.
      The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.
    • 翻訳後修飾Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
      Dephosphorylated by PTPN11.
    • 細胞内局在Cytoplasm. Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • GAB 2 antibody
      • Gab2 antibody
      • GAB2_HUMAN antibody
      • Grb 2 associated binder 2 antibody
      • GRB 2 associated binding protein 2 antibody
      • Grb2 associated binder 2 antibody
      • GRB2 associated binder 2 pp100 antibody
      • GRB2 associated binding protein 2 antibody
      • GRB2-associated binder 2 antibody
      • GRB2-associated-binding protein 2 antibody
      • Growth factor receptor bound protein 2 associated protein 2 antibody
      • Growth factor receptor bound protein 2-associated protein 2 antibody
      • KIAA0571 antibody
      • p97 antibody
      • PH domain containing adaptor molecule p97 antibody
      • pp100 antibody
      see all

    Anti-GAB2 antibody [EP369Y] 画像

    • All lanes : Anti-GAB2 antibody [EP369Y] (ab32365) at 1/500 dilution

      Lane 1 : K526 cell lysate
      Lane 2 : MCF-7 cell lysate


      Predicted band size : 74 kDa
      Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of PC-12 cells
      using ab32365 at 1/50 dilution

    Anti-GAB2 antibody [EP369Y] (ab32365) 使用論文

    This product has been referenced in:
    • Tian LQ  et al. MicroRNA-197 inhibits cell proliferation by targeting GAB2 in glioblastoma. Mol Med Rep N/A:N/A (2016). WB . Read more (PubMed: 27035789) »
    • Pyarajan S  et al. Interleukin-3 (IL-3)-induced c-fos Activation Is Modulated by Gab2-Calcineurin Interaction. J Biol Chem 283:23505-9 (2008). WB . Read more (PubMed: 18586679) »

    See all 2 Publications for this product

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    Hello! The blocking peptides for these antibodies have now been published in our catalog, they are ab133951 and ab133952. Please let me know if you have any further questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"