製品の概要

  • 製品名
    Anti-GAB1 antibody [Y423]
    GAB1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y423] to GAB1
  • 特異性
    This antibody is specific for the splice isoform 2 of human GAB1.
  • アプリケーション
    適用あり: WB, ICC/IF, Flow Cytmore details
    適用なし: IHC or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human GAB1.

  • ポジティブ・コントロール
    • WB: 293 cell lysate. ICC/IF: PC-12 cells. Flow Cyt: HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Alternative versions available:

    Anti-GAB1 antibody (Alexa Fluor® 488) [Y423] (ab208232)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 76 kDa.
ICC/IF 1/50.
Flow Cyt 1/2500.
  • 追加情報
    Is unsuitable for IHC or IP.
  • ターゲット情報

    • 機能
      Probably involved in EGF and insulin receptor signaling.
    • 配列類似性
      Belongs to the GAB family.
      Contains 1 PH domain.
    • 翻訳後修飾
      Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Gab 1 antibody
      • GAB1 antibody
      • GAB1_HUMAN antibody
      • GRB 2 associated binder 1 antibody
      • GRB 2 associated binding protein 1 antibody
      • GRB2 associated binding protein 1 isoform a antibody
      • GRB2 associated binding protein 1 isoform b antibody
      • GRB2-associated binder 1 antibody
      • GRB2-associated-binding protein 1 antibody
      • Growth factor receptor bound protein 2-associated protein 1 antibody
      see all

    画像

    • Anti-GAB1 antibody [Y423] (ab32093) at 1/5000 dilution + 293 cell lysate.

      Predicted band size : 76 kDa
      Observed band size : 76 kDa
    • ab32093 at a 1:50 dilution staining GAB1 in PC-12 cells using immunofluoresence
    • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GAB1 (red) with ab32093 at a 1/2500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

    参考文献

    This product has been referenced in:
    • Le Goff A  et al. Anti-apoptotic Role of Caspase-cleaved GAB1 Adaptor Protein in Hepatocyte Growth Factor/Scatter Factor-MET Receptor Protein Signaling. J Biol Chem 287:35382-96 (2012). WB . Read more (PubMed: 22915589) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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