The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/400 using radiolabelled second antibodies for visualization and a blocking agent such as milk. Use plasma membrane fractions of cells. Predicted molecular weight: 37 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Strongly expressed in lung and placenta, whereas it is weakly expressed in brain and heart.
Belongs to the WD repeat G protein beta family. Contains 7 WD repeats.