製品の概要

  • 製品名
    Anti-G protein alpha S antibody
    G protein alpha S 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to G protein alpha S
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide within residues: VYRATHRLLL LGAGESGKST IVKQMRILHV NGFNGEGGEE DPQAARSNSD , corresponding to N terminal amino acids 36-85 of Human G protein alpha S (NP_000507).

  • ポジティブ・コントロール
    • MCF7 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
  • 細胞内局在
    Cell Membrane
  • 参照データベース
  • 別名
    • Adenylate cyclase stimulating G alpha protein antibody
    • AHO antibody
    • Alternative gene product encoded by XL exon antibody
    • C20orf45 antibody
    • dJ309F20.1.1 antibody
    • dJ806M20.3.3 antibody
    • Extra large alphas protein antibody
    • GNAS antibody
    • GNAS complex locus antibody
    • GNAS1 antibody
    • GPSA antibody
    • Gs alpha subunit antibody
    • GSA antibody
    • GSP antibody
    • Guanine nucleotide binding protein (G protein) alpha stimulating activity polypeptide 1 antibody
    • Guanine nucleotide binding protein alpha stimulating activity polypeptide 1 antibody
    • Guanine nucleotide binding protein G(s) subunit alpha isoforms short antibody
    • Guanine nucleotide binding protein G(s) subunit alpha isoforms XLas antibody
    • Guanine nucleotide regulatory protein antibody
    • MGC33735 antibody
    • NESP antibody
    • NESP55 antibody
    • Neuroendocrine secretory protein antibody
    • PHP1A antibody
    • PHP1B antibody
    • PHP1C antibody
    • POH antibody
    • Protein ALEX antibody
    • Protein GNAS antibody
    • Protein NESP55 antibody
    • SCG6 antibody
    • Secretogranin VI antibody
    • XLalphas antibody
    • XLas antibody
    see all

画像

  • Anti-G protein alpha S antibody (ab83735) at 1 µg/ml (5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
  • ab83690 at 5 µg/ml staining G protein alpha S in Human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed, paraffin-embedded sections).
  • ab83690 at 5 µg/ml staining G protein alpha S in Human thyroid tissue by Immunohistochemistry (Formalin/PFA-fixed, paraffin-embedded sections).
  • Anti-G protein alpha S antibody (ab83735) at 1/500 dilution + murine MelJuSo cells at 20 µg

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk

    See Abreview

参考文献

This product has been referenced in:
  • Noh BJ  et al. Clinicopathological implications of GNAS in Ewing sarcoma. Oncol Lett 11:4077-4082 (2016). Read more (PubMed: 27313744) »
  • Modelska A  et al. The malignant phenotype in breast cancer is driven by eIF4A1-mediated changes in the translational landscape. Cell Death Dis 6:e1603 (2015). WB ; Human . Read more (PubMed: 25611378) »

See all 4 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - 0.3% Triton X-100
Specification
Heart
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Mrs. Aishlin Hassan

Verified customer

投稿 Jan 04 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MelJuSo)
Loading amount
20 µg
Specification
MelJuSo
Treatment
Isoprotereol
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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