Anti-G protein alpha 抗体 (ab58916)

製品の概要

  • 製品名Anti-G protein alpha antibody
    G protein alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to G protein alpha
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Cow, Pig
  • 免疫原

    A region within synthetic peptide: YRATHRLLLL GAGESGKSTI VKQMRILHVN GFNGDSEKAT KVQDIKNNLK, corresponding to N terminal amino acids 37-86 of Human G protein alpha

  • ポジティブ・コントロール
    • WB: HepG2 membrane lysate IHC-P: Human kidney tissue and human lung tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 関連性GTP-binding proteins (G-proteins)are a family of heterotrimeric proteins that play a critical role in signal transduction by coupling cell surface, 7-transmembrane domain receptors to intracellular signaling pathways including second messenger generation (such as cyclic AMP, calcium and diacylglycerol), protein phosphorylation, ion channel activation, gene induction, cell growth and differentiation. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit.
  • 細胞内局在Cell Membrane
  • 参照データベース
  • 別名
    • Adenylate cyclase stimulating G alpha protein antibody
    • AHO antibody
    • C20orf45 antibody
    • Extra large alphas protein antibody
    • GNAS complex locus antibody
    • GNAS1 antibody
    • GPSA antibody
    • GSA antibody
    • GSP antibody
    • Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 antibody
    • Guanine nucleotide binding protein G(s) alpha subunit antibody
    • Guanine nucleotide binding protein G(s) subunit alpha isoforms XLas antibody
    • guanine nucleotide regulatory protein antibody
    • NESP antibody
    • Neuroendocrine secretory protein antibody
    • PHP1A antibody
    • PHP1B antibody
    • PHP1C antibody
    • POH antibody
    • RP4-543J19.4 antibody
    • Secretogranin VI antibody
    • XLalphas antibody
    see all

Anti-G protein alpha antibody 画像

  • Anti-G protein alpha antibody (ab58916) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling G-protein alpha with ab58916 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling G-protein alpha with ab58916 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

Anti-G protein alpha antibody (ab58916) 使用論文

ab58916 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (whole brain lysate)
Loading amount 20 µg
Specification whole brain lysate
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 12 2010

Thank you for your inquiry and patience. ab58651 and ab58916 are different peptides against GNAS complex. Human Gnas1 and Gnas2 have 100% and 98% homology, respective to antibodies. The homology is very low against other subunits but the cross-reac...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"