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Purified recombinant mouse G-CSF, expressed in E. coli.
Our Abpromise guarantee covers the use of ab49960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab49960 staining G-CSF in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 7.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a Tris-base buffer. Samples were incubated with primary antibody (1/90 in PBST) for 3 hours at 25°C. A HRP-conjugated Goat anti-rat IgG polyclonal (1/90) was used as the secondary antibody.
ab49960 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"