Anti-G-CSF 抗体 [67604.111] (ab49960)

製品の概要

  • 製品名Anti-G-CSF antibody [67604.111]
    G-CSF 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [67604.111] to G-CSF
  • 特異性ab49960 neutralizes the biological activity of recombinant mouse G-CSF.
  • アプリケーション適用あり: IHC-P, WB, Neutralising, Indirect ELISAmore details
  • 種交差性
    交差種: Mouse
    非交差種: Human
  • 免疫原

    Purified recombinant mouse G-CSF, expressed in E. coli.

  • ポジティブ・コントロール
    • Mouse G-CSF
  • 特記事項Endotoxin level is < 10 ng per mg antibody as determined by the LAL method

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
WB
Neutralising
Indirect ELISA
  • 追加情報I-ELISA: Use at a concentration of 0.5 - 1 µg/ml.
    Neut: Use at a concentration of 10 - 30 mg/ml will neutralize 50% of the bioactivity due to 0.125 ng/ml recombinant mouse G-CSF using NSF-60 cells.
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 22 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
    • 配列類似性Belongs to the IL-6 superfamily.
    • 翻訳後修飾O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C17orf33 antibody
      • Colony stimulating factor 3 (granulocyte) antibody
      • Colony stimulating factor 3 antibody
      • CSF 3 antibody
      • CSF beta antibody
      • CSF3 antibody
      • CSF3_HUMAN antibody
      • CSF3OS antibody
      • Csfg antibody
      • Filgrastim antibody
      • G-CSF antibody
      • GCSA antibody
      • GCSF antibody
      • Granulocyte colony stimulating factor antibody
      • Granulocyte colony-stimulating factor antibody
      • Lenograstim antibody
      • Macrophage granulocyte inducer 2 antibody
      • MGC45931 antibody
      • MGI 2 antibody
      • Pluripoietin antibody
      see all

    Anti-G-CSF antibody [67604.111] 画像

    • ab49960 staining G-CSF in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 7.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a Tris-base buffer. Samples were incubated with primary antibody (1/90 in PBST) for 3 hours at 25°C. A HRP-conjugated Goat anti-rat IgG polyclonal (1/90) was used as the secondary antibody.

      See Abreview

    Anti-G-CSF antibody [67604.111] (ab49960) 使用論文

    ab49960 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Bone marrow-derived macrophage)
    Permeabilization Yes - Intracellular Staining Permeabilization Wash Buffer (biolegend), 0.5% Triton-X
    Specification Bone marrow-derived macrophage
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Fixation Buffer (biolegend)
    Username

    元 西躰

    Verified customer

    投稿 Feb 23 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-base Buffer
    Sample Mouse Tissue sections (Skin)
    Specification Skin
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 7.5% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Oct 01 2013

    Thank you for your patience in awaiting our reply. I have checked all of our monoclonal G-CSF antibodies and none of them have been epitope mapped, so we do not know where they bind. The polyclonal antibodies, of course, will bind many places. Please l...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"