製品の概要

  • 製品名Anti-G-CSF antibody [5D7]
    G-CSF 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5D7] to G-CSF
  • 特異性Recognizes human G-CSF. Does not react with human interleukin 3. Does not inhibit biological activity of G-CSF in vitro (See "Notes").
  • アプリケーション適用あり: WB, Sandwich ELISA, Indirect ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human recombinant granulocyte-colony stimulating factor.

  • ポジティブ・コントロール
  • 特記事項The neutralising bioassay was performed on myeloid leukemia G-NFS-60 cell line. G-CSF stimulates the proliferation of these cells in vitro. Briefly, human G-CSF was pre-incubated with monoclonal antibody 5D7 for 2 h at RT and then added to the cell suspension. Cells were cultured for 72 h. Cell proliferation was measured by MTT method. Polyclonal antibody to G-CSF and an irrelevant monoclonal antibody of the same isotype were used as controls.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名5D7
  • ミエローマunknown
  • アイソタイプIgG2a
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000.
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to G-CSF (ab9691). For sandwich ELISA, use this antibody as Capture at 1µg/ml>ab9691 as Detection.
Indirect ELISA 1/2000 - 1/20000.

ターゲット情報

  • 機能Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • 配列類似性Belongs to the IL-6 superfamily.
  • 翻訳後修飾O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C17orf33 antibody
    • Colony stimulating factor 3 (granulocyte) antibody
    • Colony stimulating factor 3 antibody
    • CSF 3 antibody
    • CSF beta antibody
    • CSF3 antibody
    • CSF3_HUMAN antibody
    • CSF3OS antibody
    • Csfg antibody
    • Filgrastim antibody
    • G-CSF antibody
    • GCSA antibody
    • GCSF antibody
    • Granulocyte colony stimulating factor antibody
    • Granulocyte colony-stimulating factor antibody
    • Lenograstim antibody
    • Macrophage granulocyte inducer 2 antibody
    • MGC45931 antibody
    • MGI 2 antibody
    • Pluripoietin antibody
    see all

Anti-G-CSF antibody [5D7] 画像

Anti-G-CSF antibody [5D7] (ab9818) 使用論文

ab9818 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience in awaiting our reply. I have checked all of our monoclonal G-CSF antibodies and none of them have been epitope mapped, so we do not know where they bind. The polyclonal antibodies, of course, will bind many places. Please l...

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Thank you for your enquiry and interest in our products. As the datasheet indicates, this antibody (ab9818: anti-G-CSF antibody) is unconjugated so it is important to use a compatible secondary antibody which is AP-linked. Currently, we do not h...

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Thank you for your email. I understand your company resells our products without our permission; although we cannot prevent this we cannot offer your customers the Abcam guarantee of refund/replacements if your customers are unhappy with the produc...

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Full-length human recombinant G-CSF was used as the immunogen.

Thank you for your enquiry. I contacted the supplier of abs 9818, 9821, and 9822 and they were able to provide me with the following information. Ab9818 is non-neutralizing. It does not inhibit the biological activity of human G-CSF in vitro. The abili...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"