製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to FZR1
  • アプリケーション
    適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: MDQDYERRLL RQIVIQNENT MPRVTEMRRT LTPASSPVSS PSKHGDRFIP SRAGANWSVN FHRINENEKS PSQNRKAKDA TSDNGKDGLA YSALLKNELL G, corresponding to amino acids 1-102 of human FZR1 (NP_057347.2), with a 26 kDa proprietary tag.

  • ポジティブ・コントロール
    • HeLa cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2, MCF-7.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 55 kDa.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
  • 組織特異性
    Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Belongs to the WD repeat CDC20/Fizzy family.
    Contains 7 WD repeats.
  • 翻訳後修飾
    Phosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint.
  • 細胞内局在
    Cytoplasm and Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDC20 like 1b antibody
    • CDC20 like protein 1 antibody
    • CDC20-like protein 1 antibody
    • CDC20C antibody
    • CDH1 antibody
    • Cdh1/Hct1 homolog antibody
    • Fizzy related protein 1 antibody
    • Fizzy related protein antibody
    • Fizzy related protein homolog antibody
    • Fizzy-related protein homolog antibody
    • Fizzy/cell division cycle 20 related 1 antibody
    • FYR antibody
    • FZR 1 antibody
    • FZR 2 antibody
    • Fzr antibody
    • FZR_HUMAN antibody
    • Fzr1 antibody
    • Fzr1 protein antibody
    • FZR2 antibody
    • HCDH 1 antibody
    • HCDH antibody
    • hCDH1 antibody
    • KIAA1242 antibody
    see all

画像

  • ICC/IF image of ab89535 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89535 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) MCF-7 cells at 10ug/ml.

  • Anti-FZR1 antibody (ab89535) at 5 µg/ml + immunogen at 0.2 µg

    Predicted band size : 55 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Anti-FZR1 antibody (ab89535) at 5 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 55 kDa
    Observed band size : 55 kDa

参考文献

This product has been referenced in:
  • Lu H  et al. The E3 ligase APC/C-Cdh1 regulates MEF2A-dependent transcription by targeting SUMO-specific protease 2 for ubiquitination and degradation. Cell Cycle 13:3892-902 (2014). WB . Read more (PubMed: 25483061) »

See 1 Publication for this product

レビューと Q&A

Application
Flow Cytometry
Sample
Mouse Cell (brain)
Permeabilization
Yes - metanol
Gating Strategy
P8
Specification
brain
Fixation
Formaldehyde
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Verified customer

投稿 Nov 17 2017

The Uniprot link on the datasheet http://www.uniprot.org/uniprot/Q9UM11 is showing FZR1. I understand that this is not enough. I apologize for the misleading information. We are looking into changing the name of the product. I have asked our a...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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