製品の概要

  • 製品名Anti-FZD3 antibody
    FZD3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FZD3
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human FZD3.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human skeletal muscle tissue lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab102965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 76 kDa).

ターゲット情報

  • 機能Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • 組織特異性Widely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis.
  • 配列類似性Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • ドメインLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • 細胞内局在Membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Frizzled 3 antibody
    • Frizzled 3 seven transmembrane spanning receptor antibody
    • Frizzled family receptor 3 antibody
    • Frizzled homolog 3 (Drosophila) antibody
    • Frizzled homolog 3 antibody
    • Frizzled-3 antibody
    • Frizzled3 antibody
    • Fz 3 antibody
    • Fz-3 antibody
    • Fz3 antibody
    • FZD 3 antibody
    • Fzd3 antibody
    • FZD3_HUMAN antibody
    • hFz 3 antibody
    • hFz3 antibody
    see all

Anti-FZD3 antibody 画像

  • Anti-FZD3 antibody (ab102965) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 76 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

Anti-FZD3 antibody (ab102965) 使用論文

ab102965 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E9.5 mouse embryo section)
Specification E9.5 mouse embryo section
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative Paraformaldehyde
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投稿 Jan 28 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"