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RabMAb

Anti-Fyn 抗体 [EPR5500] (ab125016)

製品の概要

  • 製品名
    Anti-Fyn antibody [EPR5500]
    Fyn 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5500] to Fyn
  • アプリケーション
    適用あり: WB, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide, corresponding to residues in Human Fyn.

  • ポジティブ・コントロール
    • Ramos, Jurkat, and NIH 3T3 cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

     

    Alternative versions available:

    Anti-Fyn antibody (Alexa Fluor® 647) [EPR5500] (ab207534)
    Anti-Fyn antibody (Alexa Fluor® 488) [EPR5500] (ab208391)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF 1/500.
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.
    • 組織特異性
      Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.
    • 配列類似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • 細胞内局在
      Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
    • Information by UniProt
    • 参照データベース
    • 製品の状態
      This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
    • 別名
      • C syn protooncogene antibody
      • Fyn antibody
      • FYN oncogene related to SRC FGR YES antibody
      • FYN_HUMAN antibody
      • OKT3 induced calcium influx regulator antibody
      • P59 FYN antibody
      • p59-Fyn antibody
      • Protein tyrosine kinase fyn antibody
      • Proto oncogene tyrosine protein kinase fyn antibody
      • Proto-oncogene c-Fyn antibody
      • Proto-oncogene Syn antibody
      • Protooncogene Syn antibody
      • SLK antibody
      • Src like kinase antibody
      • Src yes related novel gene antibody
      • Src-like kinase antibody
      • Src/yes related novel antibody
      • SYN antibody
      • Tyrosine kinase p59fyn T antibody
      • Tyrosine kinase p59fyn(T) antibody
      • Tyrosine-protein kinase Fyn antibody
      see all

    画像

    • All lanes : Anti-Fyn antibody [EPR5500] (ab125016) at 1/1000 dilution (purified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : HEK293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 60 kDa
      Observed band size : 60 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Fyn antibody [EPR5500] (ab125016) at 1/5000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 60 kDa
      Observed band size : 60 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunofluorescence staining of Neuro-2a cells with purified ab125016 at a working dilution of 1 in 500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. ab7291 was used to stain tubulin, and this is shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom middle and right hand panels - for the negative controls, purified ab125016 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody at a dilution of 1/500.

    • All lanes : Anti-Fyn antibody [EPR5500] (ab125016) at 1/1000 dilution (unpurified)

      Lane 1 : Ramos lysate
      Lane 2 : Jurkat lysate
      Lane 3 : NIH 3T3 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 60 kDa
      Observed band size : 59 kDa (why is the actual band size different from the predicted?)

    参考文献

    This product has been referenced in:
    • Wang G  et al. SEA antagonizes the imatinib-meditated inhibitory effects on T cell activation via the TCR signaling pathway. Biomed Res Int 2014:682010 (2014). WB ; Human . Read more (PubMed: 24524084) »

    See 1 Publication for this product

    レビューと Q&A

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jul 02 2015

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jun 27 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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