Anti-Fyn 抗体 [2A10] (ab119855)
Key features and details
- Mouse monoclonal [2A10] to Fyn
- Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-Fyn antibody [2A10]
Fyn 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2A10] to Fyn -
由来種
Mouse -
アプリケーション
適用あり: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Purified recombinant fragment of Human Fyn, expressed in E. coli.
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ポジティブ・コントロール
- IHC-P: Human breast cancer and brain tissues ICC/IF: U251 cells Flow Cyt: Hela cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
2A10 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab119855の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 61 kDa.
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ELISA |
1/10000.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/200 - 1/1000.
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特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 61 kDa. |
ELISA
1/10000. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/200 - 1/1000. |
ターゲット情報
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機能
Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3. -
組織特異性
Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
細胞内局在
Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes. - Information by UniProt
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参照データベース
- Entrez Gene: 2534 Human
- Omim: 137025 Human
- SwissProt: P06241 Human
- Unigene: 390567 Human
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製品の状態
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
別名
- C syn protooncogene antibody
- Fyn antibody
- FYN oncogene related to SRC FGR YES antibody
see all
画像
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Anti-Fyn antibody [2A10] (ab119855) at 1/500 dilution + Fyn recombinant protein (7-176 amino acids)
Predicted band size: 61 kDa
Predicted MW of Recombinant Fragment : 44.3 kDa -
ab119855, at a 1/200 dilution, staining Fyn in paraffin embedded Human breast cancer tissue by Immunohistochemistry, with DAB staining.
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ab119855, at a 1/200 dilution, staining Fyn in paraffin embedded Human brain tissue by Immunohistochemistry, with DAB staining.
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Immunofluorescence analysis of U251 cells, using ab119855 at a 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow Cytometric analysis of Hela cells, using ab119855 at a 1/200 dilution (green) and negative control (purple).
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Elisa results for ab119855
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab119855 は 1 報の論文で使用されています。
- Yamada Y et al. Identification of hypo- and hypermethylated genes related to atherosclerosis by a genome-wide analysis of DNA methylation. Int J Mol Med 33:1355-63 (2014). PubMed: 24626634