製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to FYCO1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length FYCO1 protein (Human) (AAH07218.1)

  • ポジティブ・コントロール
    • Human colon tissue lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab68914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: 1/500 - 1/1000. Detects bands of approximately 167 and 28 kDa in two separate blots (predicted molecular weight: 167 for isoform one and 29 kDa for isoforms two and three).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 組織特異性
      Expressed in heart and skeletal muscle.
    • 配列類似性
      Contains 1 FYVE-type zinc finger.
      Contains 1 GOLD domain.
      Contains 1 RUN domain.
    • 翻訳後修飾
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CATC2 antibody
      • CTRCT18 antibody
      • DKFZp779K1152 antibody
      • FLJ13335 antibody
      • FYCO1 antibody
      • FYCO1_HUMAN antibody
      • FYVE and coiled coil domain containing 1 antibody
      • FYVE and coiled coil domain containing protein 1 antibody
      • FYVE and coiled-coil domain-containing protein 1 antibody
      • MGC126517 antibody
      • MGC126519 antibody
      • RUFY3 antibody
      • RUN and FYVE domain containing 3 antibody
      • ZFYVE7 antibody
      • Zinc finger FYVE domain containing protein 7 antibody
      • Zinc finger FYVE domain-containing protein 7 antibody
      see all

    画像

    • Anti-FYCO1 antibody (ab68914) at 1/500 dilution + Human colon tissue lysate at 50 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 167 , 29 kDa
      Observed band size : 167 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 kDa,70 kDa. We are unsure as to the identity of these extra bands.
    • All lanes : Anti-FYCO1 antibody (ab68914) at 1/500 dilution

      Lane 1 : FYCO1 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 167 , 29 kDa
      Observed band size : 28 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    ab68914 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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