1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IP.
Acts as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2.
Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues.
Contains 1 SH3 domain.
T-cell receptor ligation leads to increased tyrosine phosphorylation.
ab76103, at 1/250 dilution, staining FYB in paraffin-embedded human tonsil tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-FYB antibody [EP2546Y] (ab76103)This image is courtesy of an anonymous abreview.
ICC/If image of CHO cell expressing human ADAP using ab 76103. The cells were fixed in formaldehyde and permeabilised using 0.2% Triton X100 for 5 mins. The cells were them blocked using 10% serum for 1 hour.