製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to FXR1
  • 特異性
    This antibody is expected to recognise all reported isoforms
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Dog
  • 免疫原

    Synthetic peptide:

    RIEGDNENKLPRED

    , corresponding to amino acids 317/330 of Human FXR1

  • ポジティブ・コントロール
    • NIH/3T3 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
ELISA 1/128000.

ターゲット情報

  • 機能
    RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs.
  • 組織特異性
    Expressed in all tissues examined including heart, brain, kidney and testis.
  • 配列類似性
    Belongs to the FMR1 family.
    Contains 2 KH domains.
  • 翻訳後修飾
    Arg-445 is dimethylated, probably to asymmetric dimethylarginine.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Fragile X mental retardation autosomal homolog 1 antibody
    • Fragile X mental retardation syndrome related protein 1 antibody
    • Fragile X mental retardation syndrome-related protein 1 antibody
    • FXR1 antibody
    • FXR1_HUMAN antibody
    • FXR1P antibody
    • hFXR1p antibody
    see all

Anti-FXR1 antibody 画像

  • Anti-FXR1 antibody (ab51970) at 0.2 µg/ml + NIH/3T3 cell lysate in RIPA buffer at 35 µg

    Predicted band size : 69 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab51970 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51970, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab51970 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51970, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-FXR1 antibody (ab51970) 使用論文

This product has been referenced in:
  • Oldenburg AR  et al. Deregulation of Fragile X-related protein 1 by the lipodystrophic lamin A p.R482W mutation elicits a myogenic gene expression program in preadipocytes. Hum Mol Genet N/A:N/A (2013). ICC/IF ; Human . Read more (PubMed: 24108105) »
  • Xu XL  et al. FXR1P But Not FMRP Regulates the Levels of Mammalian Brain-Specific microRNA-9 and microRNA-124. J Neurosci 31:13705-9 (2011). Read more (PubMed: 21957233) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP prostate cancer cell line)
Loading amount
20000 cells
Specification
LNCaP prostate cancer cell line
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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投稿 Oct 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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