製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FUT3
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthesized peptide derived from an internal sequence of FUT3.

  • ポジティブ・コントロール
    • Human colon carcinoma tissue; extracts from K562 cells and Jurkat cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
  • 組織特異性
    Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver.
  • パスウェイ
    Protein modification; protein glycosylation.
  • 配列類似性
    Belongs to the glycosyltransferase 10 family.
  • 細胞内局在
    Golgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha-(1,3/1,4)-fucosyltransferase antibody
    • Blood group Lewis alpha-4-fucosyltransferase antibody
    • CD174 antibody
    • FT3B antibody
    • Fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group) antibody
    • Fucosyltransferase 3 antibody
    • Fucosyltransferase III antibody
    • FucT III antibody
    • FucT-III antibody
    • FUT3 antibody
    • FUT3_HUMAN antibody
    • Galactoside 3(4)-L-fucosyltransferase antibody
    • LE antibody
    • Les antibody
    • Lewis FT antibody
    see all

Anti-FUT3 antibody 画像

  • All lanes : Anti-FUT3 antibody (ab110082) at 1/500 dilution

    Lane 1 : extracts from K562 cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from Jurkat cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 42 kDa
  • Immunohistochemical analysis of Blood Group Lewis b in paraffin embedded Human colon carcinoma tissue, using a 1/50 dilution of ab110082 with (right image) or without (left image) immunizig peptide.

Anti-FUT3 antibody (ab110082) 使用論文

ab110082 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (breast cancer MDA-MB-231)
Specification
breast cancer MDA-MB-231
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Sep 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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