Anti-Furin 抗体 - Aminoterminal end (ab28547)
製品の概要
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製品名Anti-Furin antibody - Aminoterminal end
Furin 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Furin - Aminoterminal end
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由来種Rabbit
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特異性ab28547 recognizes subtilisin-like serine protease furin.
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アプリケーション適用あり: WB, IHC-Frmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Furin (N terminal).
(Peptide available asab41397) -
ポジティブ･コントロール
- Furin is ubiquitously expressed at low levels, but found in highest concentration in the liver and kidneys, lower levels in brain, spleen, thymus, and smaller levels in most other tissues. Endogenous furin was detected in lysates from LNCaP and DU145 prostate carcinoma cell lines.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 2.9% Sodium chloride, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）This antibody has been peptide-affinity purified.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab28547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| WB | 1/1000 - 1/5000. Predicted molecular weight: 88 kDa. 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Glycosylation and other post-translational modifications make the pre-pro furin run at 110-104 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa. Dilution optimised using Chromogenic detection. |
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| IHC-Fr | Use at an assay dependent concentration. PubMed: 20454619 |
ターゲット情報
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機能Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
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組織特異性Seems to be expressed ubiquitously.
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配列類似性Belongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain. -
ドメインContains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
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翻訳後修飾The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. -
細胞内局在Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
- Information by UniProt
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参照データベース
- Entrez Gene: 5045 Human
- Entrez Gene: 18550 Mouse
- Entrez Gene: 54281 Rat
- Omim: 136950 Human
- SwissProt: P09958 Human
- SwissProt: P23188 Mouse
- SwissProt: P23377 Rat
- Unigene: 513153 Human
see all -
別名
- Dibasic processing enzyme antibody
- Dibasic-processing enzyme antibody
- FES upstream region antibody
see all
画像
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Western blot - Anti-Furin antibody - Aminoterminal end (ab28547)All lanes : Anti-Furin antibody - Aminoterminal end (ab28547) at 1/1000 dilution
Lane 1 : Furin at 0.05 µg
Lane 2 : Furin at 0.01 µg
Lane 3 : Furin at 0.001 µg
Predicted band size: 88 kDa
Observed band size: 88 kDa -
Immunohistochemistry (Frozen sections) - Anti-Furin antibody - Aminoterminal end (ab28547)Image from Hajdin K et al, PLoS One. 2010 May 3;5(5):e10445 Fig 4.ab28547 staining Furin (red) in mouse endothelial tissue by Immunohistochemistry (Frozen sections).
Mice were perfused, tumor and control organs were dissected and frozen in O.C.T. embedding medium. 10 µm cryosections were washed with PBS, fixed for 15 minutes in ice-cold methanol and air-dried. Primary antibody was used at a dilution of 1/1500. The secondary antibody was an Alexa Fluor 594-labeled IgG at a 1/300 dilution. Nuclei are visualized by DAPI staining (blue).
プロトコール
参考文献
This product has been referenced in:
- Jaaks P et al. The proprotein convertase furin is required to maintain viability of alveolar rhabdomyosarcoma cells. Oncotarget 7:76743-76755 (2016). Human . Read more (PubMed: 27572312) »
- Jaaks P et al. The Proprotein Convertase Furin Contributes to Rhabdomyosarcoma Malignancy by Promoting Vascularization, Migration and Invasion. PLoS One 11:e0161396 (2016). IHC . Read more (PubMed: 27548722) »
レビューと Q&A
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