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製品の概要

  • 製品名Anti-Furin antibody - Aminoterminal end
    Furin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Furin - Aminoterminal end
  • 特異性ab28547 recognizes subtilisin-like serine protease furin.
  • アプリケーション適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Furin (N terminal).
    (Peptide available as ab41397)

  • ポジティブ･コントロール
    • Furin is ubiquitously expressed at low levels, but found in highest concentration in the liver and kidneys, lower levels in brain, spleen, thymus, and smaller levels in most other tissues. Endogenous furin was detected in lysates from LNCaP and DU145 prostate carcinoma cell lines.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 2.9% Sodium chloride, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項（精製）This antibody has been peptide-affinity purified.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab28547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 88 kDa.

1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Glycosylation and other post-translational modifications make the pre-pro furin run at 110-104 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa. Dilution optimised using Chromogenic detection.
IHC-Fr Use at an assay dependent concentration. PubMed: 20454619

ターゲット情報

  • 機能Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
  • 組織特異性Seems to be expressed ubiquitously.
  • 配列類似性Belongs to the peptidase S8 family. Furin subfamily.
    Contains 1 homo B/P domain.
  • ドメインContains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
  • 翻訳後修飾The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
    Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.
  • 細胞内局在Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • Dibasic processing enzyme antibody
    • Dibasic-processing enzyme antibody
    • FES upstream region antibody
    • FUR antibody
    • FURIN antibody
    • Furin membrane associated receptor protein antibody
    • FURIN_HUMAN antibody
    • PACE antibody
    • Paired basic amino acid residue cleaving enzyme antibody
    • Paired basic amino acid residue-cleaving enzyme antibody
    • PCSK3 antibody
    • Proprotein convertase subtilisin/kexin type 3 antibody
    • SPC1 antibody
    see all

Anti-Furin antibody - Aminoterminal end 画像

  • Western blot - Anti-Furin antibody - Aminoterminal end (ab28547)
    Western blot - Anti-Furin antibody - Aminoterminal end (ab28547)
    All lanes : Anti-Furin antibody - Aminoterminal end (ab28547) at 1/1000 dilution

    Lane 1 : Furin at 0.05 µg
    Lane 2 : Furin at 0.01 µg
    Lane 3 : Furin at 0.001 µg


    Predicted band size : 88 kDa
    Observed band size : 88 kDa
  • Immunohistochemistry (Frozen sections) - Furin antibody - Aminoterminal end (ab28547)
    Immunohistochemistry (Frozen sections) - Furin antibody - Aminoterminal end (ab28547)Image from Hajdin K et al, PLoS One. 2010 May 3;5(5):e10445 Fig 4.
    ab28547 staining Furin (red) in mouse endothelial tissue by Immunohistochemistry (Frozen sections).
    Mice were perfused, tumor and control organs were dissected and frozen in O.C.T. embedding medium. 10 µm cryosections were washed with PBS, fixed for 15 minutes in ice-cold methanol and air-dried. Primary antibody was used at a dilution of 1/1500. The secondary antibody was an Alexa Fluor 594-labeled IgG at a 1/300 dilution. Nuclei are visualized by DAPI staining (blue).

プロトコール

Anti-Furin antibody - Aminoterminal end (ab28547) 使用論文

This product has been referenced in:
  • Jaaks P  et al. The proprotein convertase furin is required to maintain viability of alveolar rhabdomyosarcoma cells. Oncotarget 7:76743-76755 (2016). Human . Read more (PubMed: 27572312) »
  • Sathyamurthy M  et al. Characterization and expression of proprotein convertases in CHO cells: Efficient proteolytic maturation of human bone morphogenetic protein-7. Biotechnol Bioeng N/A:N/A (2014). WB . Read more (PubMed: 25219685) »

See all 4 Publications for this product

ab28547 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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