製品の概要

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab120873 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    Fura-2 AM 画像

    • Direct comparison of Abcam and competitor’s Fura-2 AM. RAW 264.7 cells were loaded with 2 µM of Fura-2 AM from Abcam (ab120873) and competitor’s in a DMSO/ HBSS solution for 30 minutes at room temperature and in the dark. After performing two washes with pre-warmed HBSS the cells were further incubated for 30 minutes at 37°C in the dark. Finally, washes with pre-warmed HBSS were performed and the live cells were imaged on a confocal microscope (using the same exposure settings): excitation laser = 405nm, emission gate center = 519nm.

    • Direct comparison of Abcam and competitor’s Fura-2 AM. RAW 264.7 cells were loaded with different concentrations of Fura-2 AM from Abcam (ab120873) and competitor’s in a DMSO/ HBSS solution for 30 minutes at room temperature and in the dark. After performing two washes with pre-warmed HBSS the cells were further incubated for 30 minutes at 37°C in the dark. Finally, washes with pre-warmed HBSS were performed and the live cells were imaged on a confocal microscope (using the same exposure settings): excitation laser = 405nm, emission gate center = 519nm.

    • RAW 264.7 cells were loaded with different concentrations of FURA2-AM (ab120873) in a pluronic F-127/DMSO/ HBSS solution for 30 minutes at room temperature and in the dark. After performing two washes with pre-warmed HBSS the cells were further incubated for 30 minutes at 37°C in the dark. Finally, washes with pre-warmed HBSS were performed and the live cells were imaged.

    Fura-2 AM (ab120873) 使用論文

    This product has been referenced in:
    • Moody TW  et al. PYK-2 is Tyrosine Phosphorylated after Activation of Pituitary Adenylate Cyclase Activating Polypeptide Receptors in Lung Cancer Cells. J Mol Neurosci 48:660-6 (2012). Read more (PubMed: 22581436) »
    • Toivari E  et al. Effects of transmitters and amyloid-beta peptide on calcium signals in rat cortical astrocytes: Fura-2AM measurements and stochastic model simulations. PLoS One 6:e17914 (2011). Read more (PubMed: 21483471) »
    • Brooke SM  et al. Autofluorescence as a confound in the determination of calcium levels in hippocampal slices using fura-2AM dye. Brain Res 706:283-8 (1996). Read more (PubMed: 8822368) »
    • Field ML  et al. Intracellular Ca2+ transients in isolated perfused rat heart: measurement using the fluorescent indicator Fura-2/AM. Cell Calcium 16:87-100 (1994). Read more (PubMed: 7982268) »

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    Excitation at 340/380nm gives maximum signal, not the 405nm that was used to collect the data shown on the datasheet, and the emission maximum wavelength is 505nm. The paper at this link, http://www.jbc.org/content/260/6/3440.long, has a good discussio...

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    Thank you for your enquiry.

    I am pleased to provide the following information for ab120873 Fura-2 AM (Fura-2 acetoxymethyl ester):

    Excitation: 340 and 380 nm.

    Emission: 505 nm

    I hope this will be helpful to y...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"