Fura-2 AM, Ca2+ selective fluorescent indicator (ab120873)
Key features and details
- High affinity Ca2+ selective fluorescent indicator
- CAS Number: 108964-32-5
- Purity: > 95%
- Soluble in DMSO to 10 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Fura-2 AM, Ca2+ selective fluorescent indicator -
製品の詳細
High affinity Ca2+ selective fluorescent indicator -
別名
- Fura 2 acetoxymethyl ester
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生理活性の詳細
High affinity Ca2+ selective fluorescent indicator (Kd = 145 nM). Rapidly metabolized by cytoplasmic esterases leading to the active dye Fura 2. Cell-permeable. Excitation spectrum dependent on [Ca2+]i. Ex340/380/Em505.
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精製度
> 95% -
特記事項
1 unit = 50 μg
- 1 mg - 1 x 1 mg vial
- 20 units - 20 x 50 µg vials
- 10 units - 10 x 50 µg vials
- 50 µg - 1 x 50 µg vial
Related protocols:
Calcium imaging using Fura-2 AM in motor neurons
Measurement of free cytosolic calcium concentration in single CHO-K1 cells
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CAS 番号
108964-32-5 -
構造式
製品の特性
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体系名
2-[6-[Bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid (acetyloxy)methyl ester -
分子量
1001.86 -
分子式
C44H47N3O24 -
PubChem 登録番号
3364574 -
保存方法
Shipped at Room Temperature. Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 10 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120873の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120873, Fura-2 AM, Ca2+ selective fluorescent indicator
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Direct comparison of Abcam and competitor’s Fura-2 AM. RAW 264.7 cells were loaded with 2 µM of Fura-2 AM from Abcam (ab120873) and competitor’s in a DMSO/ HBSS solution for 30 minutes at room temperature and in the dark. After performing two washes with pre-warmed HBSS the cells were further incubated for 30 minutes at 37°C in the dark. Finally, washes with pre-warmed HBSS were performed and the live cells were imaged on a confocal microscope (using the same exposure settings): excitation laser = 405nm, emission gate center = 519nm.
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Direct comparison of Abcam and competitor’s Fura-2 AM. RAW 264.7 cells were loaded with different concentrations of Fura-2 AM from Abcam (ab120873) and competitor’s in a DMSO/ HBSS solution for 30 minutes at room temperature and in the dark. After performing two washes with pre-warmed HBSS the cells were further incubated for 30 minutes at 37°C in the dark. Finally, washes with pre-warmed HBSS were performed and the live cells were imaged on a confocal microscope (using the same exposure settings): excitation laser = 405nm, emission gate center = 519nm.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (18)
ab120873 は 18 報の論文で使用されています。
- Wu Y et al. Human ApoE2 Endows Stronger Contractility in Rat Cardiomyocytes Enhancing Heart Function. Cells 12:N/A (2023). PubMed: 36766690
- Triantafilou M et al. Human rhinovirus promotes STING trafficking to replication organelles to promote viral replication. Nat Commun 13:1406 (2022). PubMed: 35301296
- Kai F et al. ECM dimensionality tunes actin tension to modulate endoplasmic reticulum function and spheroid phenotypes of mammary epithelial cells. EMBO J 41:e109205 (2022). PubMed: 35880301
- Jimenez-Duran G et al. Complement membrane attack complex is an immunometabolic regulator of NLRP3 activation and IL-18 secretion in human macrophages. Front Immunol 13:918551 (2022). PubMed: 36248901
- Li Z et al. PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells. Exp Ther Med 24:750 (2022). PubMed: 36561964