Anti-Fumarylacetoacetate hydrolase 抗体 (ab83770)

製品の概要

  • 製品名Anti-Fumarylacetoacetate hydrolase antibody
    Fumarylacetoacetate hydrolase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Fumarylacetoacetate hydrolase
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (SFIPVAEDSD FPIHNLPYGV FSTRGDPRPR IGVAIGDQIL DLSIIKHLFT) of Human Fumarylacetoacetate hydrolase (NP_000128)

  • ポジティブ・コントロール
    • Fetal liver lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

ターゲット情報

  • 関連性Fumarylacetoacetate hydrolase is the last enzyme in the tyrosine catabolism pathway. FAH deficiency is associated with Type 1 hereditary tyrosinemia (HT). This is an autosomal recessive inborn error of metabolism that occurs in both an acute and a chronic form. Clinical characteristics of the acute form include hepatic failure and death in infancy, whereas children with the chronic form have renal tubular dysfunction and hypophosphatemic rickets, progressive liver disease with development of hepatocellular carcinoma. Dietary treatment with restriction of tyrosine and phenylalanine alleviates the rickets, but liver transplantation has so far been the only definite treatment.
  • 参照データベース
  • 別名
    • Beta diketonase antibody
    • FAA antibody
    • FAH antibody
    • Fumarylacetoacetase antibody
    • Fumarylacetoacetate antibody
    • Fumarylacetoacetate hydrolase antibody
    see all

Anti-Fumarylacetoacetate hydrolase antibody 画像

  • Immunohistochemistry with Human liver and mouse KO tissue
  • Immunohistochemistry with Human liver and mouse KO tissue
  • Anti-Fumarylacetoacetate hydrolase antibody (ab83770) at 1 µg/ml + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Gel concentration 12%

Anti-Fumarylacetoacetate hydrolase antibody (ab83770) 使用論文

ab83770 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 30 µg
Specification liver
Gel Running Conditions Reduced Denaturing (4-20% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Dec 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"