Anti-Fumarylacetoacetate hydrolase 抗体 (ab81087)


  • 製品名Anti-Fumarylacetoacetate hydrolase antibody
    Fumarylacetoacetate hydrolase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Fumarylacetoacetate hydrolase
  • アプリケーション適用あり: ICC/IF, WB, IP, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human
  • 免疫原

    A full length recombinant protein of Fumarylacetoacetate hydrolase from Mouse origin.

  • ポジティブ・コントロール
    • Mouse liver tissue. Whole cell lysate derived from mouse liver tissue.



Our Abpromise guarantee covers the use of ab81087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 46 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P Use a concentration of 2 - 5 µg/ml.


  • 関連性Fumarylacetoacetate hydrolase is the last enzyme in the tyrosine catabolism pathway. FAH deficiency is associated with Type 1 hereditary tyrosinemia (HT). This is an autosomal recessive inborn error of metabolism that occurs in both an acute and a chronic form. Clinical characteristics of the acute form include hepatic failure and death in infancy, whereas children with the chronic form have renal tubular dysfunction and hypophosphatemic rickets, progressive liver disease with development of hepatocellular carcinoma. Dietary treatment with restriction of tyrosine and phenylalanine alleviates the rickets, but liver transplantation has so far been the only definite treatment.
  • 参照データベース
  • 別名
    • Beta diketonase antibody
    • FAA antibody
    • FAH antibody
    • Fumarylacetoacetase antibody
    • Fumarylacetoacetate antibody
    • Fumarylacetoacetate hydrolase antibody
    see all

Anti-Fumarylacetoacetate hydrolase antibody 画像

  • All lanes : Anti-Fumarylacetoacetate hydrolase antibody (ab81087) at 1/500 dilution

    Lane 1 : Whole cell lysate derived from liver extract from FAH wild type mice
    Lane 2 : Whole cell lysate derived from liver extract from FAH wild type mice
    Lane 3 : Whole cell lysate derived from FAH-liver extract from z alleles transgenic mice
    Lane 4 : Whole cell lysate derived from FAH-liver extract from z alleles transgenic mice
    Lane 5 : Whole cell lysate derived from liver extract from siLucFAH mice

    Predicted band size : 46 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
  • ab81087 at 2µg/ml staining Fumarylacetoacetate hydrolase by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded mouse liver sections), followed by HRP linked secondary antibody, and visualized by AEC substrate, and counterstaining by hematoxylin.
  • ICC/IF image of ab81087 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81087, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Fumarylacetoacetate hydrolase antibody (ab81087) 使用論文

ab81087 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Hepatocytes)
Specification Hepatocytes
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Formaldehyde

Dr. VIctoria Thompson

Verified customer

投稿 Jun 12 2013