製品の概要

  • 製品名Fumarate Detection Kit
  • テスト
    100 x 1 assay
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプQuantitative
  • 検出感度
    > 1 nmol/well
  • 全工程の試験時間
    0h 40m
  • 製品の概要

    Abcam's Fumarate Detection Kit provides a convenient tool for sensitive detection of Fumarate in a variety of samples. The Fumarate Enzyme Mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (? = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 µM).
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Fumarate (HO2CCH=CHCO2H-) is an intermediate in the Kreb's cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Fumarate (HO2CCH=CHCO2H-) is an intermediate in the Kreb’s cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy.

アプリケーション

Our Abpromise guarantee covers the use of ab102516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Fumarate Detection Kit 画像

  • Fumurate Standard Curve
  • Fumarate measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).

  • Fumarate measured in biological fluids, background signal subtracted (duplicates +/- SD).

  • Fumarate measured in cell culture medium and control medium, background signal subtracted (duplicates +/- SD).

  • Fumarate measured in cell lysates, background signal subtracted (duplicates +/- SD).

プロトコール

Fumarate Detection Kit (ab102516) 使用論文

ab102516 has not yet been referenced specifically in any publications.

Product Wall

Measurement of fumaric acid in stool

Good Good 4/5 (Ease of Use)
Abreviews
We investigated whether it was possible to measure fumaric acid in faeces. We tried several concentrations of faeces, and the results showed that it is possible to measure fumaric acid in faeces, although the measurement needs to be optimized.
Here is our protocol:
A Fumarate Detection Kit (Abcam 102516) was used for fumaric acid measurement in faeces. 40 mg of faeces was weighed and 100 ul Assay Buffer was added to the samples. Bead-beating (2 times 30 seconds) was performed, and afterwards centrifugation 10 minutes (13.000pm) for homogenization of the samples. The protein concentration of the supernatant was measured with the DC kit from Bradford Assay. Optimum protein concentration was determined testing various ug protein concentrations. After a couple try-outs, it appeared that 2.5 ul and 0.5 ul of the supernatant were the best concentrations to use in the assay. 2.5 ul and 0.5ul were both tested, and added to every well. Assay buffer was added to end up with a total concentration of 50 ul. Consequently, 100 ul reaction mix was added to each sample, which consisted of 90 ul Fumarate Assay Buffer, 8 ul Fumarate Developer and 2 ul Fumarate enzyme mix. After incubation of 60 minutes at 37°C, the absorbance was measured at 450 nm in a microplate reader.
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投稿 Jun 17 2016

As Fumarate is a chemical and not a protein, it's detection is not limited by species specificity as it would be the case if you would like to detect a protein.
In addition, as blood serum is blood plasma without clotting factors, and plasma has be...

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The final colour development should be pink but the reaction mix should not be pink to begin with. Only once you add this to your samples, will the reaction initiate and lead to the pink colour development.

Thank you for your enquiry.

I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products.

Ho...

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Thank you very much for your interest in our kit ab102516 for fumarate detection.

The laboratory confirmed that we haven’t tried this kit with microbial samples in-house and neither do we have any researcher feedback from such use. So a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"