製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FUBP1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Dog, Zebrafish
  • 免疫原

    A region within a synthetic peptide: RARQIAAKIG GDAGTSLNSN DYGYGGQKRP LEDGDQPDAK KVAPQNDSFG, corresponding to N terminal amino acids 37-86 of Human FUBP1

  • ポジティブ・コントロール
    • Fetal Brain Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.

ターゲット情報

  • 機能
    Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
  • 配列類似性
    Contains 4 KH domains.
  • 翻訳後修飾
    Ubiquitinated. This targets the protein for proteasome-mediated degradation.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA helicase V antibody
    • far upstream element (FUSE) binding protein 1 antibody
    • Far upstream element (FUSE) binding protein 4 antibody
    • Far upstream element binding protein 1 antibody
    • far upstream element binding protein antibody
    • Far upstream element-binding protein 1 antibody
    • FBP antibody
    • FUBP antibody
    • Fubp1 antibody
    • FUBP1_HUMAN antibody
    • Fubp4 antibody
    • FUSE binding protein 1 antibody
    • FUSE-binding protein 1 antibody
    • HDH V antibody
    see all

画像

  • Anti-FUBP1 antibody (ab28732) at 1.25 µg/ml + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Olanich ME  et al. Identification of FUSE-binding protein 1 as a regulatory mRNA-binding protein that represses nucleophosmin translation. Oncogene : (2010). WB ; Mouse . Read more (PubMed: 20802533) »

See 1 Publication for this product

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