The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報IP: Use at 1 - 5 µg/mg of lysate.
WB: 1/5000 - 1/25000. Detects a band of approximately 100 kDa (predicted molecular weight: 96 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
関連性FTSJD2 (FtsJ methyltransferase domain containing 2) shows RNA- and S-adenosyl-methionine binding and protein methyltransferase activity in vitro. It interacts with the C-terminal domain of RNA polymerase II. FTSJD2 may have a role in the interferon response pathway and function in the pre-mRNA processing events mediated by the C-terminal domain of the RNA polymerase II.
All lanes : Anti-FTSJD2 antibody (ab70388) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells transfected with a
myc-FTSJD2 expression construct at 15 µg Lane 2 : Whole cell lysate from 293T cells that were
mock transfected at 60 µg Lane 3 : Whole cell lysate from G361 cells at 15 µg