The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/25000. Detects a band of approximately 110 kDa (predicted molecular weight: 96 kDa).
Use at 1-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m(7)GpppG-capped mRNA and small nuclear RNA (snRNA) to produce m(7)GpppRm (cap1). Displays a preference for cap0 transcripts. Cap1 modification is linked to higher levels of translation. May be involved in the interferon response pathway.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling FTSJD2 with ab70387 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - FTSJD2 antibody (ab70387)
Detection of Human FTSJD2 by Immunoprecipitation in Whole cell lysate from 293T cells (1 mg/IP) using ab70387 at 1 µg/mg lysate for IP and at 0.1 µg/ml for subsequent WB detection.