製品の概要

  • 製品名Anti-FTSJ3 antibody
    FTSJ3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FTSJ3
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 623-654 of Human FTSJ3 (NP_060117.3).

  • ポジティブ・コントロール
    • MDA-MB231 cell line lysates. This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 97 kDa.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Probable methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation.
  • 配列類似性Belongs to the methyltransferase superfamily. RlmE family. SPB1 subfamily.
  • 細胞内局在Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2''-O-ribose RNA methyltransferase SPB1 homolog antibody
    • EPCS3 antibody
    • FLJ20062 antibody
    • FtsJ homolog 3 (E. coli) antibody
    • FTSJ3 antibody
    • pre-rRNA processing protein FTSJ3 antibody
    • Protein ftsJ homolog 3 antibody
    • Putative rRNA methyltransferase 3 antibody
    • SPB1 antibody
    • SPB1_HUMAN antibody
    see all

Anti-FTSJ3 antibody 画像

  • ICC/IF image of ab110884 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110884 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-FTSJ3 antibody (ab110884) at 1/100 dilution + MDA-MB231 cell line lysates at 35 µg

    Predicted band size : 97 kDa

Anti-FTSJ3 antibody (ab110884) 使用論文

ab110884 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab110884.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"