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RabMAb

Anti-FTO 抗体 [EPR6895] (ab124892)

製品の概要

  • 製品名
    Anti-FTO antibody [EPR6895]
    FTO 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6895] to FTO
  • アプリケーション
    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to residues in Human FTO.

  • ポジティブ・コントロール
    • Human Breast and kidney tissues; 293T, SH-SY5Y, Caco2 and BxPC3 lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • ターゲット情報

    • 機能
      Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.
    • 組織特異性
      Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
    • 関連疾患
      Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed.
    • 配列類似性
      Belongs to the fto family.
    • ドメイン
      The 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AlkB homolog 9 antibody
      • ALKBH9 antibody
      • Alpha-ketoglutarate-dependent dioxygenase FTO antibody
      • AW743446 antibody
      • Fat mass and obesity-associated protein antibody
      • FATSO, MOUSE, HOMOLOG OF antibody
      • Fto antibody
      • FTO_HUMAN antibody
      • GDFD antibody
      • KIAA1752 antibody
      • mKIAA1752 antibody
      • Protein fatso antibody
      see all

    画像



    • Predicted band size : 58 kDa

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: FTO knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HEK293 whole cell lysate (20 µg)
      Lane 4: MOLT4 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab124892 observed at 58 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab124892 was shown to specifically react with FTO in wild-type HAP1 cells. No band was observed when FTO knockout samples were examined. Wild-type and FTO knockout samples were subjected to SDS-PAGE. ab124892 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • ab124892, at 1/100 dilution, staining FTO in Paraffin-embedded Human Breast tissue by Immunohistochemistry.
    • All lanes : Anti-FTO antibody [EPR6895] (ab124892) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : Caco2 lysate
      Lane 4 : BxPC3 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 58 kDa
    • ab124892, at 1/100 dilution, staining FTO in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    参考文献

    This product has been referenced in:

    See all 6 Publications for this product

    レビューと Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HepG2)
    Permeabilization
    Yes - 0.05% Triton
    Specification
    HepG2
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Fange Liu

    Verified customer

    投稿 Mar 09 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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