製品の概要

  • 製品名Anti-FTO antibody [EPR6895]
    FTO 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6895] to FTO
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to residues in Human FTO.

  • ポジティブ・コントロール
    • Human Breast and kidney tissues; 293T, SH-SY5Y, Caco2 and BxPC3 lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for Flow Cyt,ICC/IF or IP.
  • ターゲット情報

    • 機能Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.
    • 組織特異性Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
    • 関連疾患Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed.
    • 配列類似性Belongs to the fto family.
    • ドメインThe 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AlkB homolog 9 antibody
      • ALKBH9 antibody
      • Alpha-ketoglutarate-dependent dioxygenase FTO antibody
      • AW743446 antibody
      • Fat mass and obesity-associated protein antibody
      • FATSO, MOUSE, HOMOLOG OF antibody
      • Fto antibody
      • FTO_HUMAN antibody
      • GDFD antibody
      • KIAA1752 antibody
      • mKIAA1752 antibody
      • Protein fatso antibody
      see all

    Anti-FTO antibody [EPR6895] 画像

    • All lanes : Anti-FTO antibody [EPR6895] (ab124892) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : Caco2 lysate
      Lane 4 : BxPC3 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 58 kDa
    • ab124892, at 1/100 dilution, staining FTO in Paraffin-embedded Human Breast tissue by Immunohistochemistry.
    • ab124892, at 1/100 dilution, staining FTO in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-FTO antibody [EPR6895] (ab124892) 使用論文

    This product has been referenced in:

    See all 2 Publications for this product

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