製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to FSTL1
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Cow, Cynomolgus monkey
  • 免疫原

    Synthetic peptide: TAEKTKRVSTKEI, corresponding to amino acids 296-308 of Human FSTL1.

  • ポジティブ・コントロール
    • Human Placenta and Ovary lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/16000.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human FSTL1 peptide (ab23213). The observed molecular weight corresponds to earlier findings in literature (Tanaka et al, Int Immunol. 1998 Sep;10(9):1305-14.

ターゲット情報

Anti-FSTL1 antibody 画像

プロトコール

Anti-FSTL1 antibody (ab11805) 使用論文

This product has been referenced in:
  • Sundaram GM  et al. 'See-saw' expression of microRNA-198 and FSTL1 from a single transcript in wound healing. Nature 495:103-6 (2013). Read more (PubMed: 23395958) »
  • Slany A  et al. Cell characterization by proteome profiling applied to primary hepatocytes and hepatocyte cell lines Hep-G2 and Hep-3B. J Proteome Res 9:6-21 (2010). WB ; Human . Read more (PubMed: 19678649) »

See all 4 Publications for this product

Product Wall

Thank you for your reply.

Unfortunately it won't be possible to send the replacement to your collaborator.

What about ab107431?This rabbit polyclonal antibody is directed against the C-terminal part of Human FSTL1 and the immunogen...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (mesenchymal stem cells)
Loading amount
8 µg
Specification
mesenchymal stem cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 May 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録