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In Abcam's Fructose Assay Kit, free fructose is enzymatically converted to ß-glucose, which is then specifically converted to a product that reacts with OxiRed Probe to generate color (?=570nm) and fluorescence (Ex/Em=535/587nm). The kit provides a rapid, simple, sensitive, and reliable method suitable for high throughput assayof D-fructose.
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Fructose is a monosaccharide found in many foods and is one of the three most important blood sugars along with glucose and galactose. Fructose is the sweetest naturally occurring sugar, estimated to be twice as sweet as sucrose.
|Enzyme Mix (lyophilized)||Green||1 vial|
|Fructose Assay Buffer||WM||1 x 25ml|
|Fructose Converting Enzyme (Lyophilised)||Purple||1 vial|
|Fructose Standard (100mM)||Yellow||1 x 100µl|
|OxiRed Probe in DMSO||Red||1 x 200µl|
Our Abpromise guarantee covers the use of ab83380 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Fructose levels banana, determined using the colorimetric (C) and the fluorometric (F) method (presented per mg of protein) (duplicates; +/- SD).
Fructose levels in mouse liver and banana, determined using the colorimetric method and presented per mg of protein (duplicates; +/- SD).
Titration of the fructose (background subtracted, duplicates; +/- SD).