製品の概要

  • 製品名
    Fructose Assay Kit
  • サンプルの種類
    Cell culture extracts, Tissue Extracts
  • アッセイタイプ
    Quantitative
  • 全工程の試験時間
    1h 00m
  • 製品の概要

    In Abcam's Fructose Assay Kit, free fructose is enzymatically converted to ß-glucose, which is then specifically converted to a product that reacts with OxiRed Probe to generate color (λ=570nm) and fluorescence (Ex/Em=535/587nm). The kit provides a rapid, simple, sensitive, and reliable method suitable for high throughput assayof D-fructose.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Fructose is a monosaccharide found in many foods and is one of the three most important blood sugars along with glucose and galactose. Fructose is the sweetest naturally occurring sugar, estimated to be twice as sweet as sucrose.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 ラベル 100 tests
    Enzyme Mix (lyophilized) Green 1 vial
    Fructose Assay Buffer WM 1 x 25ml
    Fructose Converting Enzyme (Lyophilised) Purple 1 vial
    Fructose Standard (100mM) Yellow 1 x 100µl
    OxiRed Probe in DMSO Red 1 x 200µl
  • 研究分野
  • 関連性
    Fructose is a monosaccharide found in many foods and is one of the three most important blood sugars along with glucose and galactose. Fructose is the sweetest naturally occurring sugar, estimated to be twice as sweet as sucrose.
  • 別名
    • Beta Levulose
    • D arabino Hexulose
    • Fruit sugar
    • Laevulose
    • Levulose
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab83380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

画像

  • Functional assays: Fructose Assay Kit (ab83380)
    Functional assays: Fructose Assay Kit (ab83380)

    Fructose levels banana, determined using the colorimetric (C) and the fluorometric (F) method (presented per mg of protein) (duplicates; +/- SD).

  • Functional assays: Fructose Assay Kit (ab83380)
    Functional assays: Fructose Assay Kit (ab83380)

    Fructose levels in mouse liver and banana, determined using the colorimetric method and presented per mg of protein (duplicates; +/- SD).

  • Functional assays: Fructose Assay Kit (ab83380)
    Functional assays: Fructose Assay Kit (ab83380)

    Titration of the fructose (background subtracted, duplicates; +/- SD).

  • Functional Studies - Fructose Assay Kit (ab83380)
    Functional Studies - Fructose Assay Kit (ab83380)
    Fluorometric Fructose Standard Curve performed following the ab83380 kit protocol.

プロトコール

参考文献

This product has been referenced in:
  • Vaughan OR  et al. A physiological increase in maternal cortisol alters uteroplacental metabolism in the pregnant ewe. J Physiol 594:6407-6418 (2016). Read more (PubMed: 27292274) »
  • Romero Mdel M  et al. Glycerol Production from Glucose and Fructose by 3T3-L1 Cells: A Mechanism of Adipocyte Defense from Excess Substrate. PLoS One 10:e0139502 (2015). Functional Studies . Read more (PubMed: 26426115) »

See all 2 Publications for this product

ab83380 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

5 reviews or Q&A

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Votes: Highest First

Is it possible to measure fructose from tissue extracts made with RIPA buffer? If so what protocol recommendations can you provide to prepare this type of sample?

Yes, samples made in RIPA buffer can be used, albeit, they need to be diluted in the respective assays buffers before use with the particular assays, so that the samples have all the components required for each reaction to occur.

Inquiry: tell the approximate cost per kit, and the number of samples which we can test at a time. waiting for reply

Thank you for contacting us. The kit is currently priced at $435 and is for 100 tests. The protocol calls for 6 readings of standards, so if you take into account doing everything in duplicate (standards and samples) that would be 44 different samples....

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Dear technical team,

Our customer would like to check protocol before ordering ab8338.
But there is no manual book on the website.
So please send me the file.
http://www.abcam.com/Fructose-Assay-Kit-ab83380.html

Bes...

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Thank you for your inquiry and for notifying us of the missing protocol.

The protocol has now been added to the datasheet and I also attached a copy for your customer to this email.

Please do not hesitate to contact us again with an...

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Thank you.  This kit should fit my needs.  Do you have a trial size version that I can test prior to ordering the full kit?

Thank you for your reply. We only have the full size kit available for purchase. However we guarantee all our products to work as stated on the datasheets, so we will be happy to help if the kit does not work as expected. Please let me kn...

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What is the detection limit of this kit?

Thanks for your call yesterday and for your question about ab83380. I have been in contact with the lab, and I was told that the detection limit when the kit is used colorimetrically (570nm) is 1-10 nmols/well. If fluorometric methods (Ex/Em = ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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