製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FRMD6
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 446-580 of Human FRMD6 (Q96NE9).

  • ポジティブ・コントロール
    • Human duodenum tissue; RT-4, EFO-21, A-431, Liver, Tonsil cell lysates; and Human cell line U-2 OS.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

ターゲット情報

画像

  • Immunohistochemical staining of human duodenum with anti-FRMD6 antibody (ab121133) at 1:350 dilution. It shows strong cytoplasmic positivity in glandular cells
  • All lanes : Anti-FRMD6 antibody (ab121133) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : EFO-21 cell lysate
    Lane 3 : A-431 cell lysate
    Lane 4 : Liver cell lysate
    Lane 5 : Tonsil cell lysate
  • Immunofluorescent staining of human cell line U-2 OS (fixed/permealized with PFA/Trion X-100) with anti-FRMD6 antibody (ab121133) at 1µg/ml in green. It shows positivity in mitochondria.

参考文献

ab121133 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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