Anti-FRMD5 抗体 (ab118737)

製品の概要

  • 製品名Anti-FRMD5 antibody
  • 製品の詳細
    Rabbit polyclonal to FRMD5
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 164-213 (FPKHSEKLEK KIAEIHKTEL SGQTPATSEL NFLRKAQTLE TYGVDPHPCK) of Rat FRMD5 (XP_230513).

  • ポジティブ・コントロール
    • Rat Muscle lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーConstituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-FRMD5 antibody 画像

  • Anti-FRMD5 antibody (ab118737) at 1 µg/ml + Rat Muscle lysate at 10 µg

    Predicted band size : 65 kDa

Anti-FRMD5 antibody (ab118737) 使用論文

ab118737 has not yet been referenced specifically in any publications.

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Thank you for submitting an Abreview for ab118737. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested species (mouse), we would like to follow up on thi...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (cerebellum)
Loading amount 100 µg
Specification cerebellum
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Fuzi Jin

Verified customer

投稿 Nov 28 2012

Thank you for your phone call.
I am sorry to hear that the antibody did not work in mouse samples. Unfortunately, we have not yet tested mouse samples and thus do not know if and how well the antibody would work.

I am sending you the disco...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"