製品の概要

  • 製品名Anti-FRMD1 antibody
    FRMD1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FRMD1
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: RYFEPHSYFP QWIITKRGID YILRHMPTLH RERQGLSPKE AMLCFIQEAC RLEDVPVHFF RLHKDKKEGR PTVILGLALR GVHIYQGKKL EI, corresponding to amino acids 205-296 of Human FRMD1.

  • ポジティブ・コントロール
    • Human bone marrow tissue Human plasma

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

ターゲット情報

  • 配列類似性Contains 1 FERM domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA164L23.1 antibody
    • DKFZp434O0117 antibody
    • FERM domain containing 1 antibody
    • FERM domain-containing protein 1 antibody
    • FLJ00181 antibody
    • FLJ22615 antibody
    • FLJ40260 antibody
    • FRMD1 antibody
    • FRMD1_HUMAN antibody
    • OTTHUMP00000219079 antibody
    • OTTHUMP00000219098 antibody
    • RP11-164L23.1 antibody
    see all

Anti-FRMD1 antibody 画像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (actin filaments). Recommended concentration of ab122109 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-FRMD1 antibody (ab122109) at 1/250 dilution

    Lane 1 : RT 4 cell line lysate
    Lane 2 : U 251 MG cell line lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate
  • ab122109, at 1/20 staining FRMD1 in paraffin embedded Human bone marrow by Immunohistochemistry.

Anti-FRMD1 antibody (ab122109) 使用論文

ab122109 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Human Cell (Saos2 Human Osteosarcoma)
Specification Saos2 Human Osteosarcoma
Permeabilization Yes - TBST
Fixative Paraformaldehyde
Username

jonathan larochelle

Verified customer

投稿 Apr 14 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"