製品の概要

  • 製品名Anti-Frizzled 9 antibody
    Frizzled 9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Frizzled 9
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Guinea pig
  • 免疫原

    Synthetic peptide within residues: TRNDPHALCM EAPENATAGP AEPHKGLGML PVAPRPARPP GDLGPGAGGS , corresponding to N terminal amino acids 144-193 of Human Frizzled 9 (NP_003499)

  • ポジティブ・コントロール
    • Fetal brain lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

ターゲット情報

  • 機能Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • 組織特異性Expressed predominantly in adult and fetal brain, testis, eye, skeletal muscle and kidney. Moderately expressed in pancreas, thyroid, adrenal cortex, small intestine and stomach. Detected in fetal liver and kidney.
  • 配列類似性Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • ドメインLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 349 antibody
    • CD 349 antigen antibody
    • CD349 antibody
    • Frizzled Drosophila homolog of 3 antibody
    • Frizzled homolog 9 (Drosophila) antibody
    • Frizzled homolog 9 antibody
    • Frizzled-9 antibody
    • Fz 9 antibody
    • Fz-9 antibody
    • FZD 3 antibody
    • Fzd 9 antibody
    • FZD3 antibody
    • FZD3-PEN antibody
    • FZD9 antibody
    • FZD9_HUMAN antibody
    • FzE6 antibody
    • hFz9 antibody
    • mfz9 antibody
    • Xfz9 antibody
    see all

Anti-Frizzled 9 antibody 画像

  • Anti-Frizzled 9 antibody (ab83744) at 1 µg/ml (in 5% skim milk/ PBS) + Fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 64 kDa

Anti-Frizzled 9 antibody (ab83744) 使用論文

ab83744 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"