製品の概要

  • 製品名Anti-Frizzled 9 antibody
    Frizzled 9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Frizzled 9
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Rabbit, Cow, Dog, Human, Pig, Chimpanzee, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Mouse Frizzled 9 aa 550 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9R216

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Mouse tissue lysates: E12 Embryo Brain and Spinal cord; Eye; Testis.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab102956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).

ターゲット情報

  • 機能Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • 組織特異性Expressed predominantly in adult and fetal brain, testis, eye, skeletal muscle and kidney. Moderately expressed in pancreas, thyroid, adrenal cortex, small intestine and stomach. Detected in fetal liver and kidney.
  • 配列類似性Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • ドメインLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 349 antibody
    • CD 349 antigen antibody
    • CD349 antibody
    • Frizzled Drosophila homolog of 3 antibody
    • Frizzled homolog 9 (Drosophila) antibody
    • Frizzled homolog 9 antibody
    • Frizzled-9 antibody
    • Fz 9 antibody
    • Fz-9 antibody
    • FZD 3 antibody
    • Fzd 9 antibody
    • FZD3 antibody
    • FZD3-PEN antibody
    • FZD9 antibody
    • FZD9_HUMAN antibody
    • FzE6 antibody
    • hFz9 antibody
    • mfz9 antibody
    • Xfz9 antibody
    see all

Anti-Frizzled 9 antibody 画像

  • All lanes : Anti-Frizzled 9 antibody (ab102956) at 1 µg/ml

    Lane 1 : E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Eye (Mouse) Whole Cell Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa (possible non-specific binding),50 kDa (possible non-specific binding).

    Exposure time : 2 minutes

    Frizzled 9 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab102956 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-Frizzled 9 antibody (ab102956) 使用論文

ab102956 has not yet been referenced specifically in any publications.

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